[Cytometry] CD14 positive cell line
PPham at nybloodcenter.org
Wed Feb 19 10:08:48 EST 2014
Have you looked into THP-1 cell lines, such as THP1-XBlue(tm)-CD14 or other commercially available lines?
(I am not affiliated to any cell line manufacturers)
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Geza Paukovics
Sent: Tuesday, February 18, 2014 7:26 PM
To: Nuno Oliveira
Cc: cytometry at lists.purdue.edu
Subject: Re: [Cytometry] CD14 positive cell line
>From memory U937 is a monocytic cell line, just cross check if it constitutively expresses CD14. A quick google search showed that vitamin D3 enhanced CD14 expression in these cells, but I didn't read enough into it whether it's constitutive.
Hope that helps if you do not have ready access to primary monocytes.
On Wed, Feb 19, 2014 at 7:56 AM, Nuno Oliveira <cravista at gmail.com> wrote:
> If I understood your question, the answer is mature monocytes. Almost
> all of them express CD14.
> Nuno Oliveira, IPOCFG - Flow Cytometry Division
> 2014-02-18 13:56 GMT+00:00 Vyzasatya Ravi - Lonza Walkersville <
> ravi.vyzasatya at lonza.com>:
> > Dear Flowers,
> > Do you know of any cell line(s) that constitutively express CD14? I
> > am looking for a CD14 cell line that I can use as positive control
> > in a flow assay.
> > Thanks in advance.
> > Ravi
> > Ravi Vyzasatya
> > Scientist
> > Cell Therapy Development Services
> > Lonza Walkersville, Inc.
> > 8830 Biggs Ford Road
> > US - 21793-0127 Walkersville
> > Tel. : +1 301 378 1152
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