[Cytometry] CD14 positive cell line

Vyzasatya Ravi - Lonza Walkersville ravi.vyzasatya at lonza.com
Wed Feb 19 11:59:32 EST 2014


Exactly..
Tried THP-1. It is negative for CD14.
As Tracey Duff suggested Mono Mac 6 may be my best bet.

RV

From: Ina Laura Pieper [mailto:inalaurapieper at gmail.com]
Sent: Wednesday, February 19, 2014 4:52 AM
To: Nuno Oliveira
Cc: Vyzasatya Ravi - Walkersville; cytometry at lists.purdue.edu
Subject: Re: [Cytometry] CD14 positive cell line

Do mature monocytes proliferate in culture though? I think Ravi may be looking for a cell line that can be maintained and harvested whenever a biological CD14+ control is needed for flow?
I've got daily access to blood samples so I use the CD14+ monocytes in whole blood as a control, but I would be very interested in learning more about CD14+ cell lines too.

Best wishes
Ina Laura

On 18 February 2014 20:56, Nuno Oliveira <cravista at gmail.com<mailto:cravista at gmail.com>> wrote:
Hi

If I understood your question, the answer is mature monocytes. Almost all
of them express CD14.

Greetings,

Nuno Oliveira, IPOCFG - Flow Cytometry Division


2014-02-18 13:56 GMT+00:00 Vyzasatya Ravi - Lonza Walkersville <
ravi.vyzasatya at lonza.com<mailto:ravi.vyzasatya at lonza.com>>:

> Dear Flowers,
>
> Do you know of any cell line(s) that constitutively express CD14? I am
> looking for a CD14 cell line that I can use as positive control in a flow
> assay.
>
> Thanks in advance.
> Ravi
>
> Ravi Vyzasatya
> Scientist
> Cell Therapy Development Services
> Lonza Walkersville, Inc.
> 8830 Biggs Ford Road
> US - 21793-0127 Walkersville
> Tel. : +1 301 378 1152<tel:%2B1%20301%20378%201152>
>
>
>
>
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