[Cytometry] CD14 positive cell line

Nuno Oliveira cravista at gmail.com
Tue Feb 18 18:43:29 EST 2014


Hi

Yes, I forgot that aspect. CD64 is much better of course,but CD14 was the
asked marker, and as far as I know, monocytes seems to be the best cell
line, even heterogeneous.... If you know another line more, I would like to
know as well.

Greetings

Nuno Oliveira, IPOCFG - Flow Cytometry Division


2014-02-18 23:39 GMT+00:00 Miller, David <Milled3 at labcorp.com>:

> Normal, human, peripheral blood monocytes are much less homogeneous in
> their expression of CD14 than you might think. If you gate on CD64 x SSC,
> you will see that the monocytes have a long tail of CD14 expression....dtm
>
> -----Original Message-----
> From: cytometry-bounces at lists.purdue.edu [mailto:
> cytometry-bounces at lists.purdue.edu] On Behalf Of Nuno Oliveira
> Sent: Tuesday, February 18, 2014 3:57 PM
> To: Vyzasatya Ravi - Lonza Walkersville; cytometry at lists.purdue.edu
> Subject: Re: [Cytometry] CD14 positive cell line
>
> Hi
>
> If I understood your question, the answer is mature monocytes. Almost all
> of them express CD14.
>
> Greetings,
>
> Nuno Oliveira, IPOCFG - Flow Cytometry Division
>
>
> 2014-02-18 13:56 GMT+00:00 Vyzasatya Ravi - Lonza Walkersville <
> ravi.vyzasatya at lonza.com>:
>
> > Dear Flowers,
> >
> > Do you know of any cell line(s) that constitutively express CD14? I am
> > looking for a CD14 cell line that I can use as positive control in a
> > flow assay.
> >
> > Thanks in advance.
> > Ravi
> >
> > Ravi Vyzasatya
> > Scientist
> > Cell Therapy Development Services
> > Lonza Walkersville, Inc.
> > 8830 Biggs Ford Road
> > US - 21793-0127 Walkersville
> > Tel. : +1 301 378 1152
> >
> >
> >
> >
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