[Cytometry] Consistent difference between CST vs Manual laser delays
Chuan En Lam
chuanen.lam at gmail.com
Tue Feb 18 19:21:39 EST 2014
Recently we seem to be seeing consistent differences between CST set laser
delay values vs Manual laser delay values on our AriaIIu.
AriaIIu (Blue/Red/Violet laser) inside ClassI CAS hood.
Morning cold temp: 20.0deg,
Start up to CST: 22.0 deg
2hrs after start up to end of day: about 24.0 deg
Mostly, after initial fluidics startup, CST would be run. We then double
check with manual laser delay setting using single peak beads and 8 peak
We found a consistent requirement to shift the laser delays by about 0.5
compared to CST to get optimum position. This is in general consistent
across nozzles (70um, 100um). We are not sure if temp changes has an
effect, or was the flow cell dirty (have done flow cell cleans with
In one scenario, 70um nozzle, after flow cell clean.
_________CST AM____Manual AM____12PM____4PM____4pmCST____4pmManual
Red -43.06 -43.50 -43.50 -43.50
Violet 40.64 40.18 39.92
39.92 40.46 39.86
Has others seen similar effects or ideas what this might indicate?
Normal condition sorting (log scales etc) seems to be ok with clean
Flow Cytometry Lab Technician
Garvan Institute of Medical Research
Ph 9295 8431
e.lam at garvan.org.au <r.salomon at garvan.org.au>
More information about the Cytometry