[Cytometry] whole blood

Marta Mesa mmesa at javeriana.edu.co
Tue Feb 18 11:17:56 EST 2014


Hi Barbara,

OptiLyse® BLysing Solution Beckman-Coulter PN IM1400 works quite well. 
I have used it with nice results in the  phenotyping of circulating leukocytes from children and adults with no lyse no wash protocol.

Best regards,

Marta Mesa
Bogotá, Colombia



On Feb 7, 2014, at 4:03 AM, barbara morandi wrote:

> Dear all,
> I would like to know whether someone can suggest how to perform immunofluorescence staining on whole blood (CD3, CD56, CD16, CD19, CD14). I've tried to lyse red cells with BD FACS lysis solution but it seems it doesn't work very well. Can anyone suggest a protocol to efficiently lyse red cells?
> Thanks a lot,
> Barbara
>  
> Dr. Barbara Morandi
> Immunology Unit
> Istituto Nazionale per la Ricerca sul Cancro
> CBA- L.go R.Benzi, 10
> 16132 - Genova, Italy
> Tel :39 (010) 5558-221
> Fax: 39 (010) 354282
> E-mail: morandibarb at yahoo.it_______________________________________________
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