[Cytometry] 2nd ESCCA International Summer School on Cytometry

Enrique O'Connor jose.e.oconnor at uv.es
Sun Feb 9 10:37:31 EST 2014


Dear Flowers:
I have the pleasure of inviting you to 
participate in the 2nd edition of the 
International Summer School on Cytometry, in 
Valencia (Spain), 7-11 and 14-18 July 2014.
Best regards
Enrique O'Connor

SECOND INTERNATIONAL SUMMER SCHOOL ON CYTOMETRY
www.escca.eu/meetings/summer-school
Organized by European Society for Clinical Cell 
Analysis (ESCCA), University of Valencia, 
Principe Felipe Research Center and Sociedad Iberica de Citometria

  7-14 and 14-18 JULY 2014 in VALENCIA, SPAIN

The International Summer School on Cytometry 
(ISSC) is a program of basic and specialized 
courses created by the Educational Committee of 
ESCCA. The first edition of the ISSC was held 
successfully in Valencia, Spain on July 2013, and 
brought together 57 students (from 22 countries) 
and 33 teachers (from 16 countries).

The second edition of the ISSC will be held on 
7-18 July 2014, and will consist of two series of 
two parallel 5-day courses starting from Monday 
morning to Friday afternoon and limited to 30 
students per course. The courses will present 
methods and applications of Cytometry and 
Cytomics in basic, clinical and translational 
fields, and will approach practical aspects of 
assay design and performance, data analysis and 
interpretation, using commercially available 
cytometers, reagents and software. Attendees 
registering for two courses in separate weeks 
will benefit of 20% discount in the registration fee.

Theoretical lectures and technical seminars 
delivered by international experts will alternate 
with extensive practical exercises in wet labs 
and computer rooms. The ISSC courses, taught in 
English, are informal, open and convivial, and 
include social activities. Although all the ISSC 
courses are open to people active in Cytometry, 
experienced or specialized cytometrists will 
benefit better from the specialized courses. All 
the Courses cover data analysis, use and 
comparison of cytometry software, and real data 
review, being thus suitable also for those responsible for final results.

ISCC offers a limited number of Travel 
Fellowships (150 €) to participants from 
developing countries or from countries not having 
a local cytometry society. To this purpose, a 
motivation letter should be addressed to 
José-Enrique O’Connor (jose.e.oconnor at uv.es) 
and  Claude Lambert (claude.lambert at chu-st-etienne.fr).

REGISTRATION (www.escca.eu/meetings/summer-school)
  (On-line opening: February 15, 2014; Deadline 
for early registration: May 31, 2014)
COURSE(S) ONLY
·       ESCCA MEMBERS:
-       Before May 31, 2014: 550 €
-       Late registration (Until June 30, 2014): 650 €
Includes Course materials, lunches and coffees, 
Welcome and Gala Dinners and Social activities.
·       NON ESCCA MEMBERS:
-       Before May 31, 2014: 650 €
-       Late registration (Until June 30, 2014): 750 €
Includes Course materials, lunches and coffee, 
Welcome and Gala Dinners and Social activities 
plus one-year full membership in ESCCA. Attendees 
registering for two courses in separate weeks 
will benefit of 20% discount in the registration fee.
ACCOMMODATION
-       Before May 31, 2014: 350 €
-       Late reservation  (Only until June 15, 2014): 450 €
Includes 6-night lodging (Sunday-Friday) at bed & 
breakfast rate in a University Residence, at 15 
min walking distance from venue. Additional 
nights may be requested to the organization.

OVERVIEW OF COURSE CONTENTS

FUNCTIONAL CYTOMETRY: UNDERSTANDING AND APPLYING 
CYTOMIC ASSAYS (7-11 July 2014. Level: Basic)
Summary: Overview of Single-cell technologies 
(Flow cytometry, Mass-spectrometry cytometry; 
Cell Sorting, Image-in-Flow Cytometry, 
High-Content analysis by Bioimaging). Overview of 
applications of functional Cytometry and 
Cytomics. Functional assays of cell health (Cell 
cycle and cell proliferation; Drug metabolism; 
Drug effects; Cell death). Functional assays of 
cell communication and activation (extracellular 
and intracellular signaling). Functional assays 
of cell response (Cytoenzimology; Phagocytosis; 
Oxidative responses; Microvesicle release; 
Secretion of extracellular molecules; 
Cytotoxicity). Analysis of microorganisms. 
Practicals in wet lab and computer room.

FLOW CYTOMETRY IN HEMATOLOGY: A TECHNICAL 
APPROACH (7-11 July 2014. Level: Basic)
Summary: Overview of applications of cytometry in 
Hematology. Sample collection and preparation 
procedures: Blood, bone marrow, lymph nodes and 
special samples. Multiparametric immunophenotype: 
Settings, compensations and gating strategies. 
Data management: Acquisition, analysis, display 
and interpretation. Assay standardization and 
validation. Quality control. Case-oriented 
immunophenotypic studies in wet lab and computer 
room: Normal Hematopoiesis; Erythrocytes and 
erythroid cells; Acute and chronic leukemias; 
Lymphomas; Myelodysplastic syndromes; Analysis of 
rare cells and clones; Detection of minimal 
residual disease. Case-oriented functional 
studies in wet lab and computer room: Paroxysmal 
Nocturnal Haemoglobinuria; Drug resistance; Stem 
cells; Platelets and microvesicles.

CYTOMICS IN DRUG RESEARCH AND TOXICOLOGY: A 
FUNCTIONAL APPROACH (14-18 July 2014; Level: Specialized)
Summary: Introduction to Cell-Based methods in 
Pharmacology and Toxicology. Overview of cytomic 
technologies (Flow cytometry, Mass-spectrometry 
cytometry; Cell Sorting, Image-in-Flow Cytometry, 
High-Content analysis by Bioimaging). Overview of 
applications of Cytomics in drug research, drug 
discovery and Toxicology. Cytomic analysis of the 
stages and consequences of the drug-cell 
interactions (Receptor biology and 
receptor-mediated drug effects; Drug transport 
across membranes; Intracellular drug metabolism; 
Intracellular drug effects; Cell lesion and cell 
death). High-Content Cytomics. High-Troughput 
Cytomics. Cytomic assays for general and 
organ-specific toxicity (Immunotoxicity; 
Hematotoxicity; Genotoxicity; Endocrine 
disruption; Ecotoxicity). Cytomics and predictive 
Toxicology. Integration of Cytomics in 
preclinical  and regulatory strategies for drug 
safety and chemical risk assessment. Practicals in wet lab and computer room.

CYTOMETRY AND STEM CELLS: TRANSLATIONAL RESEARCH 
AND THERAPY (14-18 July 2014; Level: Specialized)
Summary: Overview of the basic biology of Stem 
cells. Overview of the clinical relevance of Stem 
cells. Overview of Cytometry applications in Stem 
Cell Research and therapy. Cytometric methods for 
Stem Cell study: Identification and phenotypic 
characterization; Functional characterization; 
Purification of Stem Cell subpopulations. 
Cytometry in basic and translational studies of 
Stem Cells: Embryonic and induced pluripotent 
stem cells; Hematopoietic stem cells; Mesenchymal 
stem cells; Cancer stem cells. Cytometry in 
clinical studies of Stem Cells: Enumeration and 
isolation of circulating Stem Cells; Assessment 
of cell therapeutic products; Monitoring of Stem 
Cell therapy and Tissue Regeneration. Practicals in wet lab and computer room.

José-Enrique O'Connor, Ph.D.
Catedrático
Departamento de Bioquímica y Biología Molecular
Facultad de Medicina de la Universidad de Valencia
Avda. Blasco Ibáñez, 17
46010 Valencia, Spain
Phone: +34 963 864988
Fax: +34 963 864001
twitter:@CytoSummer





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