[Cytometry] 6 color leukemia/lymphoma panels

Nuno Oliveira cravista at gmail.com
Mon Feb 10 17:30:14 EST 2014


Hi

I never had such expreience. Otherwise FMC7 is a B cell marker and you
always need at least 2 backbone markers for all tubes. I normally use CD19
and CD20. I have a lot of cases both positives, since FMC7 is negative or
low just in LLC, some folicular lymphomas and some large B cell lymphomas.
Mantle cell lymphoma, MALT lymphoma, and specially splenic lymphoma of
marginal zone should be FMC7+/+++ with CD20+/++.

Greetings,

Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal


2014-02-10 20:53 GMT+00:00 Peters, Avis <Avis.Peters at allina.com>:

> I have a question on your second tube. I was under the assumption that
> FMC7 and CD20 could not be put into the same tube or the FMC7 will come up
> negative due to competition for binding sites between the two antibodies.
> Is that correct?
>
> Avis Peters MLS(ASCP) QCYM
> Flow Cytometry Technical Specialist
> Allina Health Laboratory
> Minneapolis, MN
> 612-863-0555
> avis.peters at allina.com
>
> -----Original Message-----
> From: cytometry-bounces at lists.purdue.edu [mailto:
> cytometry-bounces at lists.purdue.edu] On Behalf Of Nuno Oliveira
> Sent: Saturday, February 08, 2014 4:45 AM
> To: Pam Woodall; cytometry at lists.purdue.edu
> Subject: Re: [Cytometry] 6 color leukemia/lymphoma panels
>
> Dear Pam,
>
> A small correction for tube 1 in T cell pathology:
> 1. 26 / 28 / 3 / 27 / 8 / 4
> I would used this and the same tube 1 of B cell pathology for screening
> (8+L / 56+K / 3 / 20+4 / 19+GD / 5), before entering this panel. Once you
> know that you are facing T cell pathology, then I would enter this "T
> panel".
>
> Thanks
>
> Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal
>
>
>
> 2014-02-08 10:40 GMT+00:00 Nuno Oliveira <cravista at gmail.com>:
>
> > Dear Pam,
> >
> >
> > Hi don´t know any panel with 6 colours used by someone, at least in
> > Portugal, which is my country.  However I can write down 2 panels that
> > should be suitable to use.
> >
> > For B cell pathology:
> > 1. 8+L / 56+K / 3 / 20+4 / 19+GD / 5
> > 2. FMC7 / 23 / 20 / 22 / 19 / 24
> > 3. IgM / 10 / 20 / 38 / 19 / 27 *
> > 4. 43 / 200 / 20 / 79b / 19 / 27 *
> > 5. 62L / 81 / 20 / 95 / 19 / 27 *
> > 6. 31 / 305 / 20 / 103 / 19 / 11c
> > Notes: I use 5 colour panels. Sometimes I need CD25 to identify some
> > lymphomas just when a lot of options are already out of question. CD24
> > is not on the Euroflow panel. If may help but it is not really
> > necessary. From the Euroflow panel just CD39, CXCR5 and CD49d are out. I
> don´t use them.
> > FMC7 is also not on the Euroflow panel. However I still use it because
> > it can help in some lymphomas. HLA-DR is on the Euroflow panel, but
> > also not really necessary. CD27 could take part of tubes 3, 4 or 5.
> > Maybe it gives more information if you use it in tube 3.
> >
> > For T cell pathology:
> > 1. 8 / 28 / 3 / 27 / GD / 4
> > 2. 2 / 7 / 3 / 5 / 8 / 4
> > 3. 57 / 56 / 3 / 11b / 8 / 4
> > 4. 16 / 94 / 3 / 11c / 8 / 4
> > 5. 45RA / 45RO / 3 / 8 / CC7 / 4
> > 6. HLA-DR / 11a / 3 / 38 / 8 / 4
> > 7. 62L / TCL1 / 3 / 25 / 8 / 4
> > Notes: The last tube would be used only to catch T-Prolymphocytic
> > Leukemia. CD11a is also not part of the Euroflow panel, but some
> > groups still use it. I think it happens the same with CD25. I never
> > used CD279a to catch angioimmunoblastic lymphoma, because I never had a
> case.
> >
> >
> > For leukemia panel I would have to think a little bit more, since I
> > don´t have a lot of cases, so I just have a screening panel.
> >
> >
> > Greetings,
> >
> > Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal
> >
> >
> >
> >
> > 2014-02-06 17:41 GMT+00:00 Pam Woodall <Pam.Woodall at chhi.org>:
> >
> >> We are a clinical laboratory that currently does 4 color and some 5
> >> color flow cytometry for leukemia and lymphomas.  We are currently
> >> bringing up 6 color.  We are looking for examples of panels that have
> >> been found useful in the clinical setting. Thank you for any
> >> suggestions.
> >>
> >> Pam
> >>
> >>
> >>
> >> Pamela Woodall MT(ASCP)
> >>
> >> Section Chief Immunohematology
> >>
> >> Cabell Huntington Hospital
> >>
> >> 1340 Hal Greer Blvd..
> >>
> >> Huntington, WV 25701
> >>
> >> 304-526-2517 fas 304-526-2187
> >>
> >> pam.woodall at chhi.org
> >>
> >>
> >>
> >>
> >>
> >> Confidentiality: The information contained in this communication is
> >> confidential and/or privileged, proprietary information that is
> >> transmitted solely for the purpose of the intended recipient(s). If
> >> the reader of this message is not an intended recipient, or if this
> >> message has been inadvertently directed to your attention, you are
> >> hereby notified that you have received this communication and any
> >> attached
> >> document(s) in error, and that any review, dissemination,
> >> distribution or copying of this communication is strictly prohibited.
> >> If you received this communication in error, please do the following:
> >> (1) contact me immediately at 1-304-526-2517 (2) return the email to
> >> pam.woodall at chhi.org; and (3) delete the email from your system.
> >>
> >>
> >>
> >>
> >> _______________________________________________
> >> Cytometry mailing list
> >> Cytometry at lists.purdue.edu
> >> https://lists.purdue.edu/mailman/listinfo/cytometry
> >> Search the list archive at  http://tinyurl.com/cytometry
> >>
> >>
> >
> This message contains information that is confidential and may be
> privileged. Unless you are the addressee (or authorized to receive for the
> addressee), you may not use, copy or disclose to anyone the message or any
> information contained in the message. If you have received the message in
> error, please advise the sender by reply e-mail and delete the message.
>
>


More information about the Cytometry mailing list