[Cytometry] 6 color leukemia/lymphoma panels

Peters, Avis Avis.Peters at allina.com
Mon Feb 10 15:53:09 EST 2014


I have a question on your second tube. I was under the assumption that FMC7 and CD20 could not be put into the same tube or the FMC7 will come up negative due to competition for binding sites between the two antibodies. Is that correct?

Avis Peters MLS(ASCP) QCYM
Flow Cytometry Technical Specialist
Allina Health Laboratory
Minneapolis, MN
612-863-0555
avis.peters at allina.com

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Nuno Oliveira
Sent: Saturday, February 08, 2014 4:45 AM
To: Pam Woodall; cytometry at lists.purdue.edu
Subject: Re: [Cytometry] 6 color leukemia/lymphoma panels

Dear Pam,

A small correction for tube 1 in T cell pathology:
1. 26 / 28 / 3 / 27 / 8 / 4
I would used this and the same tube 1 of B cell pathology for screening (8+L / 56+K / 3 / 20+4 / 19+GD / 5), before entering this panel. Once you know that you are facing T cell pathology, then I would enter this "T panel".

Thanks

Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal



2014-02-08 10:40 GMT+00:00 Nuno Oliveira <cravista at gmail.com>:

> Dear Pam,
>
>
> Hi don´t know any panel with 6 colours used by someone, at least in 
> Portugal, which is my country.  However I can write down 2 panels that 
> should be suitable to use.
>
> For B cell pathology:
> 1. 8+L / 56+K / 3 / 20+4 / 19+GD / 5
> 2. FMC7 / 23 / 20 / 22 / 19 / 24
> 3. IgM / 10 / 20 / 38 / 19 / 27 *
> 4. 43 / 200 / 20 / 79b / 19 / 27 *
> 5. 62L / 81 / 20 / 95 / 19 / 27 *
> 6. 31 / 305 / 20 / 103 / 19 / 11c
> Notes: I use 5 colour panels. Sometimes I need CD25 to identify some 
> lymphomas just when a lot of options are already out of question. CD24 
> is not on the Euroflow panel. If may help but it is not really 
> necessary. From the Euroflow panel just CD39, CXCR5 and CD49d are out. I don´t use them.
> FMC7 is also not on the Euroflow panel. However I still use it because 
> it can help in some lymphomas. HLA-DR is on the Euroflow panel, but 
> also not really necessary. CD27 could take part of tubes 3, 4 or 5. 
> Maybe it gives more information if you use it in tube 3.
>
> For T cell pathology:
> 1. 8 / 28 / 3 / 27 / GD / 4
> 2. 2 / 7 / 3 / 5 / 8 / 4
> 3. 57 / 56 / 3 / 11b / 8 / 4
> 4. 16 / 94 / 3 / 11c / 8 / 4
> 5. 45RA / 45RO / 3 / 8 / CC7 / 4
> 6. HLA-DR / 11a / 3 / 38 / 8 / 4
> 7. 62L / TCL1 / 3 / 25 / 8 / 4
> Notes: The last tube would be used only to catch T-Prolymphocytic 
> Leukemia. CD11a is also not part of the Euroflow panel, but some 
> groups still use it. I think it happens the same with CD25. I never 
> used CD279a to catch angioimmunoblastic lymphoma, because I never had a case.
>
>
> For leukemia panel I would have to think a little bit more, since I 
> don´t have a lot of cases, so I just have a screening panel.
>
>
> Greetings,
>
> Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal
>
>
>
>
> 2014-02-06 17:41 GMT+00:00 Pam Woodall <Pam.Woodall at chhi.org>:
>
>> We are a clinical laboratory that currently does 4 color and some 5 
>> color flow cytometry for leukemia and lymphomas.  We are currently 
>> bringing up 6 color.  We are looking for examples of panels that have 
>> been found useful in the clinical setting. Thank you for any 
>> suggestions.
>>
>> Pam
>>
>>
>>
>> Pamela Woodall MT(ASCP)
>>
>> Section Chief Immunohematology
>>
>> Cabell Huntington Hospital
>>
>> 1340 Hal Greer Blvd..
>>
>> Huntington, WV 25701
>>
>> 304-526-2517 fas 304-526-2187
>>
>> pam.woodall at chhi.org
>>
>>
>>
>>
>>
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