[Cytometry] 6 color leukemia/lymphoma panels

Nuno Oliveira cravista at gmail.com
Sat Feb 8 05:46:15 EST 2014


Another note: Instead of CC7 we should read CCR7.

Thanks

Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal



2014-02-08 10:44 GMT+00:00 Nuno Oliveira <cravista at gmail.com>:

> Dear Pam,
>
> A small correction for tube 1 in T cell pathology:
> 1. 26 / 28 / 3 / 27 / 8 / 4
> I would used this and the same tube 1 of B cell pathology for screening
> (8+L / 56+K / 3 / 20+4 / 19+GD / 5), before entering this panel. Once you
> know that you are facing T cell pathology, then I would enter this "T
> panel".
>
> Thanks
>
> Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal
>
>
>
> 2014-02-08 10:40 GMT+00:00 Nuno Oliveira <cravista at gmail.com>:
>
> Dear Pam,
>>
>>
>> Hi don´t know any panel with 6 colours used by someone, at least in
>> Portugal, which is my country.  However I can write down 2 panels that
>> should be suitable to use.
>>
>> For B cell pathology:
>> 1. 8+L / 56+K / 3 / 20+4 / 19+GD / 5
>> 2. FMC7 / 23 / 20 / 22 / 19 / 24
>> 3. IgM / 10 / 20 / 38 / 19 / 27 *
>> 4. 43 / 200 / 20 / 79b / 19 / 27 *
>> 5. 62L / 81 / 20 / 95 / 19 / 27 *
>> 6. 31 / 305 / 20 / 103 / 19 / 11c
>> Notes: I use 5 colour panels. Sometimes I need CD25 to identify some
>> lymphomas just when a lot of options are already out of question. CD24 is
>> not on the Euroflow panel. If may help but it is not really necessary. From
>> the Euroflow panel just CD39, CXCR5 and CD49d are out. I don´t use them.
>> FMC7 is also not on the Euroflow panel. However I still use it because it
>> can help in some lymphomas. HLA-DR is on the Euroflow panel, but also not
>> really necessary. CD27 could take part of tubes 3, 4 or 5. Maybe it gives
>> more information if you use it in tube 3.
>>
>> For T cell pathology:
>> 1. 8 / 28 / 3 / 27 / GD / 4
>> 2. 2 / 7 / 3 / 5 / 8 / 4
>> 3. 57 / 56 / 3 / 11b / 8 / 4
>> 4. 16 / 94 / 3 / 11c / 8 / 4
>> 5. 45RA / 45RO / 3 / 8 / CC7 / 4
>> 6. HLA-DR / 11a / 3 / 38 / 8 / 4
>> 7. 62L / TCL1 / 3 / 25 / 8 / 4
>> Notes: The last tube would be used only to catch T-Prolymphocytic
>> Leukemia. CD11a is also not part of the Euroflow panel, but some groups
>> still use it. I think it happens the same with CD25. I never used CD279a to
>> catch angioimmunoblastic lymphoma, because I never had a case.
>>
>>
>> For leukemia panel I would have to think a little bit more, since I don´t
>> have a lot of cases, so I just have a screening panel.
>>
>>
>> Greetings,
>>
>> Nuno Oliveira, Flow Cytometry division - IPOCFG, Portugal
>>
>>
>>
>>
>> 2014-02-06 17:41 GMT+00:00 Pam Woodall <Pam.Woodall at chhi.org>:
>>
>>> We are a clinical laboratory that currently does 4 color and some 5
>>> color flow cytometry for leukemia and lymphomas.  We are currently
>>> bringing up 6 color.  We are looking for examples of panels that have
>>> been found useful in the clinical setting. Thank you for any
>>> suggestions.
>>>
>>> Pam
>>>
>>>
>>>
>>> Pamela Woodall MT(ASCP)
>>>
>>> Section Chief Immunohematology
>>>
>>> Cabell Huntington Hospital
>>>
>>> 1340 Hal Greer Blvd..
>>>
>>> Huntington, WV 25701
>>>
>>> 304-526-2517 fas 304-526-2187
>>>
>>> pam.woodall at chhi.org
>>>
>>>
>>>
>>>
>>>
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>>>
>>>
>>>
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>>
>


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