[Cytometry] whole blood

barbara morandi morandibarb at yahoo.it
Fri Feb 7 04:03:31 EST 2014


Dear all,
I would like to know whether someone can suggest how to perform immunofluorescence staining on whole blood (CD3, CD56, CD16, CD19, CD14). I've tried to lyse red cells with BD FACS lysis solution but it seems it doesn't work very well. Can anyone suggest a protocol to efficiently lyse red cells?
Thanks a lot,
Barbara
 
Dr. Barbara Morandi
Immunology Unit
Istituto Nazionale per la Ricerca sul Cancro
CBA- L.go R.Benzi, 10
16132 - Genova, Italy
Tel :39 (010) 5558-221
Fax: 39 (010) 354282
E-mail: morandibarb at yahoo.it


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