[Cytometry] Aria I vs LSRII bacteria--fluorescence analysis

Nebe-Von-Caron, G g.nebe-von-caron at alere.com
Wed Feb 5 18:02:08 EST 2014


Send me the two comparable listmode files and I have a quick look but they should give you similar results indeed if you get it right. 



Gerhard Nebe-von-Caron  

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Erin Kelly
Sent: 05 February 2014 20:52
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Aria I vs LSRII bacteria--fluorescence analysis

Hello all,


This is a message from my lab mate we'd really appreciate some insight!

I am trying to use the flow cytometer to analyze fluoresce levels from a repressed system by varying concentrations of the signalling molecule. There are two flow cytometers available : aria I and the lsr II.
I would like to know if either of the machines have any particular advantage over the other with respect to my experiment ( sensitivity to varied fluorescence signals, reliability of data).

I have been receiving conflicting information about this.  The guy in charge of the machines says the LSR II is better for fluorescence readings, but apparently they Aria I is more sensitive to small cells (I'm working with E. coli).  Additionally, I tested samples on the same day on both the LSR II and the Aria and the results look the same.

Thanks



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