[Cytometry] BC Navios or BD FACSCanto II ?
marie c bene
mariecbene at gmail.com
Tue Feb 4 02:49:34 EST 2014
Dear Te Chich
As you can read in F Solly's paper, if you want to harmonize a Canto II and
a Navios, you have to use a factor of 256 to calculate the target
flourescence of the beads or cells that you are using for this purpose.
Your second question was very recently answered by F Preijers who published
just this study in Cytometry part B.
2014-02-04 Te Chih LIU <te_chih_liu at nuhs.edu.sg>:
> Dr Bene,
> Thank you for your comment.
> With regards to the harmonization procedure, your paper indicated that a
> "correction factor' had to be applied to the signals to make the plots
> similar. Can this be easily done, 'automatically / on the fly' by the
> analysis software or do the fcs files have to be manually converted prior
> to analysis.
> Additionally, have you tried running the Euroflow panels on the Navios and
> compared the plots / analyses?
> We have been running the Euroflow on the Canto 2 for the last 4 years
> quite happily but would like to keep an open mind in case we need to switch
> Te-Chih Liu,
> National University Hospital, Singapore
> -----Original Message-----
> From: cytometry-bounces at lists.purdue.edu [mailto:
> cytometry-bounces at lists.purdue.edu] On Behalf Of marie c bene
> Sent: Friday, January 31, 2014 11:18 PM
> To: cytometry at lists.purdue.edu
> Subject: Re: [Cytometry] BC Navios or BD FACSCanto II ?
> Hi Pal
> Both Canto II and Navios are good instruments and you might be interested
> in a paper our French group published recently about harmonization between
> them (Solly F et al. Cytometry part A) Navios differs by a better
> discrimination of fluorescence at low intensities linked to the higher
> number of bits (easily demonstrated by the broad CVs of dim beads or cells
> in the first two decades on a Canto), by providing 12 parameters (10 FL),
> being equipped with a carousel with single tube vortexing before
> acquisition (important for large batches or long acquisition times i.e.
> single platform absolute counts or minimal residual disease , see Lambert C
> Cyometry part B, 2005), excellent photoprotection for fragile
> fluorochromes, very stable thermostated flow cell and lasers), and finally
> no light loss with UV.
> Hope this will help you.
> MC Béné
Pr Marie Christine Béné
CHU de Nantes
9 Quai Moncousu
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