[Cytometry] DyeCycle violet
Eric.Massicotte at ircm.qc.ca
Tue Feb 4 11:33:00 EST 2014
I wanted to thank everyone who responded to my query. The discussion was very helpful.
It turns out that we chose to sort the different fractions of GFP and analyze them by PI stain (in citrate buffer with detergent and RNAse).
Besides that, we will also give a try with the Verapamil for stopping dye efflux from the MRD or ABCG2 pumps.
Thanks again to all of you,
Responsable, Cytométrie en flux
Manager, Flow cytometry
Institut de Recherches cliniques de Montréal (IRCM)
110 ave. des Pins Ouest
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