[Cytometry] CD10 clone "issue" : clincal flow question.
flowmail at verizon.net
Sat Feb 1 11:52:26 EST 2014
Thanks for the clarification, Nuno. More markers = better definition!
Back to the original posting, it seems clear from responses and brief literature search that 5&10 double positive cases exist and shouldn't be discounted as "incorrect" antibody. Brian's issue with one clone positive and one clone negative points to a technical not biological issue with that vial of that clone in that lab during that test. Hopefully he resolves it and can control for it in future panels.
On Feb 1, 2014, at 2:26 AM, Nuno Oliveira <cravista at gmail.com> wrote:
> Not all hairy cell leukemias have CD5+ and CD10+. Normally both should be
> negative. In some cases, one or both could appear positive. You can also
> use LAIR1 and CD200 to help on this. Both very strong means hairy cell
> leukemia with CD20 strong among other markers...
> Yours sincerely,
> Nuno Oliveira, IPOCFG Portugal - Flow Cytometry division
> 2014-01-31 Cris Bare <flowmail at verizon.net>:
>> From years ago I have this notion that CD5 CD10 double positive is hairy
>> cell lymphoma. The name stuck with me.
>> In the panel are you running single color anti-CD10 tubes or multicolor?
>> Could there be a setup issue where one or the other wasn't properly gated
>> or controlled?
>> Personally I'd agree with you that stopping the use of one clone over one
>> case is a bit rushed. But I'm also not an MD.
>> On Thu, Jan 30, 2014 at 7:58 AM, Marsiglia, Brian <
>> Brian.Marsiglia at inova.org
>>> I had an interesting case this week. It was a tissue that was CD5, CD10
>>> positive (B cell Lymphoma). I went back and reset the CD10 tubes and got
>>> the same result. In my panel I have two CD10 clones, which are also
>>> different fluorochromes. The CD10 in FITC (ALB 2 clone) was CD10
>>> and the CD10 PC5 (ALB 1 clone) was CD10 positive.
>>> The clinician involved in this case has ordered immunostains to verify
>>> which is "correct" .Clinically this case goes with a CD5 positive
>>> immunophentype. It was also requested that I stop using whichever clone
>>> the "incorrect" clone, confirmed my immunostains.
>>> I'm not a fan of this idea.
>>> I've done a good deal of reading on this topic, and it looks like there
>>> are cases where the lymphomas can be positive for both.
>>> Any feedback on this situation would be appreciated.
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