[Cytometry] CD5 in a lymphoproliferative panel

Eshel Batya batya.e at ziv.health.gov.il
Thu Apr 25 02:45:55 EDT 2013


Coulter's BL1a is also IgG2a, so that's not the problem. I also get much higher backgrounds with BD's clone L17512 CD5 PerCP-cy5.5 than with Coulter's CD5 PE clone L17512.


Elizabeth Eshel
Flow Cytometry Unit
Hematology Laboratories Room 209
Ziv Medical Center
Safed, Israel
tel (w) +972-(0)4-682-8151
     (c) +972-(0)50-220-1367
fax +972-(0)4-682-8851


-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Mikhail Roshal
Sent: Thursday, April 25, 2013 1:22 AM
To: Dalal, Bakul [Dr.][VA]; 'cytometry at flowcyt.cyto.purdue.edu'
Subject: Re: [Cytometry] CD5 in a lymphoproliferative panel

Hi Dr Bakul,
BL1a (Coulter) works reasonably well as does UCHT2 (BD). It may have something to do with the your clone being IgG2a (C1q interaction with another IgG2 is a possibility) Misha

Misha Roshal MD, PhD
Assistant Professor,
Department of Pathology and Laboratory Medicine Weill-Cornell Medical College.

On 4/24/13 12:04 PM, "Dalal, Bakul [Dr.][VA]" <Bakul.Dalal at vch.ca> wrote:

>We have recently experienced high bkg in CD5 using clone L17512. We use
>CD5 APC, PE-Cy7 and PerCP-Cy5.5, and all three give the same high bkg 
>(APC least bkg > PerCP-Cy5.5 > PE-Cy7). Can anyone share his/her 
>experience?
>
>Do you have a clone that works well?
>
>Many thanks.
>
>Bakul I. Dalal MD FRCPC FCAP FASCP
>Clinical Professor, Faculty of Medicine, Univ British Columbia 
>Director, Flow Cytometry Service Division of Hematopathology, Vancouver 
>General Hospital Vancouver, BC, Canada 
>bakul.dalal at vch.ca<mailto:bakul.dalal at vch.ca> / 
>BakulDalal at gmail.com<mailto:BakulDalal at gmail.com> / +1 (604) 875-4496 
>(O)
>/+1 604 779 6968 (C)
>
>
>
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