[Cytometry] CD5 in a lymphoproliferative panel

Mikhail Roshal mir9079 at med.cornell.edu
Wed Apr 24 18:22:01 EDT 2013


Hi Dr Bakul,
BL1a (Coulter) works reasonably well as does UCHT2 (BD). It may have
something to do with the your clone being IgG2a (C1q interaction with
another IgG2 is a possibility)
Misha

Misha Roshal MD, PhD
Assistant Professor,
Department of Pathology and Laboratory Medicine
Weill-Cornell Medical College.

On 4/24/13 12:04 PM, "Dalal, Bakul [Dr.][VA]" <Bakul.Dalal at vch.ca> wrote:

>We have recently experienced high bkg in CD5 using clone L17512. We use
>CD5 APC, PE-Cy7 and PerCP-Cy5.5, and all three give the same high bkg
>(APC least bkg > PerCP-Cy5.5 > PE-Cy7). Can anyone share his/her
>experience?
>
>Do you have a clone that works well?
>
>Many thanks.
>
>Bakul I. Dalal MD FRCPC FCAP FASCP
>Clinical Professor, Faculty of Medicine, Univ British Columbia
>Director, Flow Cytometry Service
>Division of Hematopathology, Vancouver General Hospital
>Vancouver, BC, Canada
>bakul.dalal at vch.ca<mailto:bakul.dalal at vch.ca> /
>BakulDalal at gmail.com<mailto:BakulDalal at gmail.com> / +1 (604) 875-4496 (O)
>/+1 604 779 6968 (C)
>
>
>
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