[Cytometry] monocyte-platelet aggregates

D. Robert Sutherland rob.sutherland at utoronto.ca
Fri Apr 19 10:01:51 EDT 2013


Adeeb,
I am not an expert in tis area, but I believe EDTA is the anti-coagulant of choice for the types of studies you are doing.
HTH
best
rob

D. Robert Sutherland
Toronto General Hospital/University Health Network

On 2013-04-18, at 4:11 PM, Adeeb Rahman wrote:

> Dear flow folk,
> 
> Do any of you have experience detecting monocyte-platelet aggregates by flow? I've been trying to quantify MPAs using antibodies against CD14 (clone HCD14) and CD42b (clone HIP1) to stain whole blood, followed by RBC lysis/fixation with BD FACSLyse.Using this protocol, I'm finding that a very high frequency of monocytes are CD42b+ (over 90%), which is much higher than the frequency reported in various publications (typically ~10%). 
> 
> 
> I've looked at blood collected in citrate tubes and sodium heparin tubes and have looked at tubes drawn later in the draw sequence (i.e. not the first tube drawn) but am still seeing the same thing. Does anyone have any thoughts about what may be causing this high frequency of MPAs? 
> Thanks,
> 
> Adeeb
> 
>  
> ___________________________
> 
> 
> Adeeb Rahman
> Postdoctoral Fellow
> Division of Liver Diseases
> Mount Sinai School of Medicine
> New York, NY
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