[Cytometry] COPAS (or similar) large particle sorter

Ian Dimmick ian.dimmick at newcastle.ac.uk
Fri Apr 19 09:44:36 EDT 2013


If the particles are big enough you can use buckets

:)



Ian 


>-----Original Message-----
>From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-
>bounces at lists.purdue.edu] On Behalf Of Rachael Walker
>Sent: 19 April 2013 14:18
>To: Pulak, Rock
>Cc: cytometry at lists.purdue.edu
>Subject: Re: [Cytometry] COPAS (or similar) large particle sorter
>
>Hi
>
>By coincidence to this thread, I was told this morning by a scientist I was
>showing around the lab, that there are modules available to add onto existing
>sorters to sort large particles such as c.elegans.  Does anyone know if there
>are such modules available?
>
> I thought on a conventional sorter you can increase a nozzle size up to 200um
>and if want to sort anything larger you need a Copas.
>
>Thanks
>
>Rachael
>
>
>Sent from my iPad
>
>On 19 Apr 2013, at 14:04, "Pulak, Rock" <rpulak at unionbio.com> wrote:
>
>> Hi Rosemary and Vanta,
>>
>> The Aria is OK for eggs/embryos and the first larval stage. Anything bigger
>(second, third, fourth and Adults stages) starts to become too big for the 100
>micron nozzle and the generation of droplets for sorting. Also, there are a lot
>of other particles, (for example, the cuticles of molts) that can clog the small
>100 micron flow cell. Despite these limitations, it does work well for that one
>early slice of the animal's life cycle. The larger flow cells of the COPAS
>instruments and the method of sorting, overcome these shortcomings.
>>
>> Best regards,
>>
>> Rock Pulak
>> Union Biometrica, Inc.
>>
>> -----Original Message-----
>> From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-
>bounces at lists.purdue.edu] On Behalf Of Vanta Jameson
>> Sent: Wednesday, April 17, 2013 8:55 PM
>> To: Rosemary Clarke; cytometry at lists.purdue.edu
>> Subject: Re: [Cytometry] COPAS (or similar) large particle sorter
>>
>> Hi Rosemary
>>
>> I'm not UK based so can't help you there.  I have had a similar enquiry to sort
>c elegans on my Aria III, and the researcher sent this Nature Protocols
>reference where they sort on an Aria I using 100um at 20psi, just lowing the
>drop drive.
>>
>> Fernandez, A. G., Bargmann, B. O. R., Mis, E. K., Edgley, M. L., Birnbaum, K.
>D., & Piano, F. (2012). High-throughput fluorescence-based isolation of live C.
>elegans larvae. Nature Protocols, 7(8), 1502-1510. doi:10.1038/nprot.2012.084
>>
>> We have not yet tried it, but it may work for sorting the smallest larval stage
>nematodes.
>>
>>
>> good luck!
>>
>> Vanta
>>
>>
>> ________________________________________
>> From: cytometry-bounces at lists.purdue.edu [cytometry-
>bounces at lists.purdue.edu] on behalf of Rosemary Clarke
>[r.z.clarke at dundee.ac.uk]
>> Sent: Wednesday, 17 April 2013 5:44 PM
>> To: cytometry at lists.purdue.edu
>> Subject: [Cytometry] COPAS (or similar) large particle sorter
>>
>> Dear All,
>>
>> I have been asked by a colleague to investigate the availability of large
>particle sorters here in the UK. Specifically it would be used for sorting
>nematode worms. If there is anyone on the list who knows of a suitable
>instrument in the UK please let me know.
>>
>> Thanks
>> Rosie
>>
>> Dr Rosie Clarke
>> Flow Cytometry Facility Manager
>> WTB/MSI
>> University of Dundee
>> Dundee
>> Scotland
>> UK
>> DD1 5EH
>> Tel: (01382) 385817
>> E-mail: r.z.clarke at dundee.ac.uk
>>
>> The University of Dundee is a registered Scottish Charity, No: SC015096
>>
>>
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