[Cytometry] COPAS (or similar) large particle sorter
rpulak at unionbio.com
Thu Apr 18 07:40:51 EDT 2013
Hi Rosemary and Vanta,
The Aria is OK for eggs/embryos and the first larval stage. Anything bigger (second, third, fourth and Adults stages) starts to become too big for the 100 micron nozzle and the generation of droplets for sorting. Also, there are a lot of other particles, (for example, the cuticles of molts) that can clog the small 100 micron flow cell. Despite these limitations, it does work well for that one early slice of the animal's life cycle. The larger flow cells of the COPAS instruments and the method of sorting, overcome these shortcomings.
Union Biometrica, Inc.
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Vanta Jameson
Sent: Wednesday, April 17, 2013 8:55 PM
To: Rosemary Clarke; cytometry at lists.purdue.edu
Subject: Re: [Cytometry] COPAS (or similar) large particle sorter
I'm not UK based so can't help you there. I have had a similar enquiry to sort c elegans on my Aria III, and the researcher sent this Nature Protocols reference where they sort on an Aria I using 100um at 20psi, just lowing the drop drive.
Fernandez, A. G., Bargmann, B. O. R., Mis, E. K., Edgley, M. L., Birnbaum, K. D., & Piano, F. (2012). High-throughput fluorescence-based isolation of live C. elegans larvae. Nature Protocols, 7(8), 1502-1510. doi:10.1038/nprot.2012.084
We have not yet tried it, but it may work for sorting the smallest larval stage nematodes.
From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] on behalf of Rosemary Clarke [r.z.clarke at dundee.ac.uk]
Sent: Wednesday, 17 April 2013 5:44 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] COPAS (or similar) large particle sorter
I have been asked by a colleague to investigate the availability of large particle sorters here in the UK. Specifically it would be used for sorting nematode worms. If there is anyone on the list who knows of a suitable instrument in the UK please let me know.
Dr Rosie Clarke
Flow Cytometry Facility Manager
University of Dundee
Tel: (01382) 385817
E-mail: r.z.clarke at dundee.ac.uk
The University of Dundee is a registered Scottish Charity, No: SC015096
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