[Cytometry] COPAS (or similar) large particle sorter
vanta.jameson at unimelb.edu.au
Wed Apr 17 20:55:04 EDT 2013
I'm not UK based so can't help you there. I have had a similar enquiry to sort c elegans on my Aria III, and the researcher sent this Nature Protocols reference where they sort on an Aria I using 100um at 20psi, just lowing the drop drive.
Fernandez, A. G., Bargmann, B. O. R., Mis, E. K., Edgley, M. L., Birnbaum, K. D., & Piano, F. (2012). High-throughput fluorescence-based isolation of live C. elegans larvae. Nature Protocols, 7(8), 1502–1510. doi:10.1038/nprot.2012.084
We have not yet tried it, but it may work for sorting the smallest larval stage nematodes.
From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] on behalf of Rosemary Clarke [r.z.clarke at dundee.ac.uk]
Sent: Wednesday, 17 April 2013 5:44 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] COPAS (or similar) large particle sorter
I have been asked by a colleague to investigate the availability of large particle sorters here in the UK. Specifically it would be used for sorting nematode worms. If there is anyone on the list who knows of a suitable instrument in the UK please let me know.
Dr Rosie Clarke
Flow Cytometry Facility Manager
University of Dundee
Tel: (01382) 385817
E-mail: r.z.clarke at dundee.ac.uk
The University of Dundee is a registered Scottish Charity, No: SC015096
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