[Cytometry] Custom BV510 filter for Gallios

Suzanne Mertens suzanne.mertens at gmail.com
Wed Apr 17 10:01:30 EDT 2013

Hellos Chris,
Here's a question: when using non-Coulter fluoros in conjunction with
optimized filtersets, what is to be done when performing QC using FlowSet
Pro? I've heard that the original filterset needs to be swapped in for
QC... can you confirm or clarify?

On Tue, Apr 16, 2013 at 9:39 AM, Chris Carpenter <thecarpe at gmail.com> wrote:

> If you have any specific applications questions about the Gallios/Navios,
> I'll be happy to help find answers.  I work for Beckman Coulter in the Life
> Sciences division.
> On Mon, Apr 15, 2013 at 7:56 PM, Kelly Lundsten <klundsten at biolegend.com>wrote:
>> Hi Suzanne!
>> How are you?  You have a 550/40 or so right now for Krome Orange in that
>> position?  The BV510 will still be really strong with that filter but you
>> are right, the peak is at 520-ish to be ideal.  Since you don't have a
>> trigon on the Gallios, it's just a position for BV421 and BV510 (right?),
>> you can use a 520/40nm filter to get the most out of the BV510.  We just
>> released another 5 BV510 conjugates just today, so that list will grow fast
>> and will also be conjugated to the harder to detect markers like CD197 and
>> CD117, etc. If you were detecting the BV510 on a trigon/octagon so that the
>> BV510 was wedged between BV421 and BV570, then you might not want a big
>> 40nm bandpass since the BV570 spillover would be annoying.
>> Our experience in testing the filters for all of the Brilliant Violets
>> was an interesting one.  Half that were sent were promiscuous to the laser
>> light and didn't pass CST.  In my experience, trial and error is the only
>> way to sort out good filters from inadequate.
>> Sincerely,
>> Kelly
>> -----Original Message-----
>> From: cytometry-bounces at lists.purdue.edu [mailto:
>> cytometry-bounces at lists.purdue.edu] On Behalf Of Suzanne Mertens
>> Sent: Monday, April 15, 2013 9:52 AM
>> To: cytometry at lists.purdue.edu
>> Subject: [Cytometry] Custom BV510 filter for Gallios
>> Hellos,
>> I'm working to get a filter set optimized for Biolegends' BV510. Does
>> anyone have any special experiences doing this? I've done this before on an
>> LSRII for the various PE tandems, so no big deal to work with Chroma - I
>> just wanted to hear if anyone else has done this for the Gallios/Navios.
>> thank-you,
>> PS. This is a shout-out to Ed Podniesinski: Your ICCS newsletter
>> contribution on optical filters is awesome!
>> http://www.cytometry.org/public/newsletters/eICCS-4-1/article3.php
>> --
>> - suzanne
>> ------------------------------------------
>> suzanne.mertens at gmail.com
>> 404-337-1533
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> --
> Chris Carpenter
> http://about.me/carpe

- suzanne

suzanne.mertens at gmail.com

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