[Cytometry] Forward Scatter Issues with BD CantoII
bhanks at usouthal.edu
Tue Apr 16 15:54:10 EDT 2013
Thanks to all who responded to my inquiry.
I focused mostly on the fluidic pathway and was reminded to check the optical pathway for cleanliness also.
Per BD FSE Craig Hayes, I carefully cleaned the prisms and lenses with ETOH and lens paper. With a lint free Q-tip and hemostats, I also gently wiped the sides of the flowcell itself. This yielded a huge improvement in the 1.00 um bead FSC-A histogram.
Another suggestion to decrease the Window Extension, (under the Laser tab), from 7 to 2 was very helpful.
Again, thanks to all who contribute to this website and make it such a treasure of information.
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