[Cytometry] Formation of a new core

Jennifer Uhrlaub juhrlaub at email.arizona.edu
Sat Apr 13 16:55:21 EDT 2013

Hi Lorelle
Reading all these suggestions I want to stress the importance of knowing 
what Biosafety level you will need to operate at to meet the needs of 
your users.   BSL2 (including running human cells) will almost certainly 
be a requirement and taking things to BSL3 requires quite a lot of 
infrastructure and space.  Do not rely on your institutions Biosafety 
office, talk directly to people who have labs set-up similarly to what 
you will need.
Good Luck!

Jennifer Uhrlaub
Laboratory Manager

University of Arizona
Department of Immunobiology
On 4/12/2013 2:22 PM, Maria Godinho Duarte Soares wrote:
> Dear Lorelle,
> A lot has been said already that you really must consider. A designated area for cell sorters, with adequate health and safety issues seen to is absolutely critical, ideally with external air supply for the wetcarts. The noise created by the built in compressors will get the sorter operator rundown by the end of the day. Do not forget liquid  waste disposal sites closeby and enough space for backup autoclaved sheath nearby. We have a microscope on the bench, but I don´t think its absolutely critical.
> The Microscopy Unit at our institute is independent from us, but we cooperate quite well whenever required.
> We have a separate area with mac workstations for data analysis that is essential, especially for those users who don´t use flow that much and don´t have the analysis software in their labs.
> I would strongly advise that your office should be as close as possible to the instruments. If I had the space I would have my office inside the lab (physically separated by glass for example). It will allow you to keep an eye on users and also on your staff, being able to help out whenever required while being able to do your office work.
> Allow enough space for liquid handling (sheath for the analyser, cleaning solutions,etc). This will be particularly important if you will be preparing your own solutions that will have to be available for users 24-7.
> Make sure that the air inside the flow lab has enough turnover. The reason I am saying this is because in our lab we only have a small entry point and the air gets really saturated. Good air quality for a room with lots of equipment and users is a must.
> Something that I haven´t seen mentioned is the installation of a UPS.
>   If you expect to have external users to come over to use your instruments have some bench space available, if you can to allow them to stain just before using the instruments.
> Lastly, plan ahead in terms of space. We flowers can quickly become popular and you may require additional instruments sooner than you think!
> Good Luck!
> Maria
> --------------------------------------------
> Maria Soares
> PhD
> Head of the Flow Cytometry Unit
> Instituto de Medicina Molecular
> Av. Prof Egas Moniz,
> 1649-028 Lisboa
> Portugal
> Lab phone: + 351 21 7999530
> x 4722, 47224
> Email:  msoares at fm.ul.pt , mariasoares100 at yahoo.co.uk
> ________________________________________
> From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] On Behalf Of David Coder [davecoder at gmail.com]
> Sent: 12 April 2013 17:51
> To: lparker at chori.org
> Cc: cytometry at lists.purdue.edu
> Subject: Re: [Cytometry] Formation of a new core
> Hi Lorelle,
> As others have mentioned, planning *before* having to build is essential.
> Having modified and built (from the ground up) core facilities at the Fred
> Hutchinson Cancer Research Center, there can be learning curve. (I say this
> as probably the only cytometrist who also has a degree in architecture.)
> There should be help from architects/engineers who deal with laboratory
> space--but they will not have all the answers as cytometry and sorting have
> special requirements.
> There are a wide range of issues from utilities (don't overlook secure,
> high speed internet), to room access, light levels, transfer hoods, sample
> prep/storage/disposal, ergonomics of workstations and instrument placement,
>   building location (heating, ventilation, air conditioning, vibrations from
> outside),  biosafety (both operator protection and product protection),
> ease of instrument access for service--to name some.
> I'd be happy to talk further if you wish.
> *David M. Coder, PhD*
> Irvine, CA
> Email: DaveCoder at gmail.com
> Cell phone: 949 233 2641
> Skype: dave.coder
>> ---------- Forwarded message ----------
>> From: "Simon Monard" <monard at wehi.EDU.AU>
>> To: "Nicolas Loof" <Nicolas.Loof at utsouthwestern.edu>
>> Cc: cytometry at lists.purdue.edu
>> Date: Fri, 12 Apr 2013 12:20:56 +1000 (EST)
>> Subject: Re: [Cytometry] Formation of a new core
>> Hi Lorelle
>> There is quite a lot to consider, if you are going to Cyto 2013 there will
>> be lots of people to talk to about this, you should visit a few labs.  You
>> don't say whether you'll have cell sorters, I assume so. Is the room
>> established already? I also assume you'll be sorting human cells in which
>> case your facility should probably  be divided into sorting and analysis
>> sections with the sorters in a lab which is BL3 or close to BL3. Thats not
>> something you can easily retrofit so should be established right away. If
>> your instruments will be in hoods will the ceiling height be high enough?
>> You'll need all the other facilities such as air, vacuum etc as Nicolas
>> says. Save yourself a whole lot of grief later on and get the lab built
>> with biosafety in mind.
>> Best
>> Simon
>>> Hello Lorelle,
>>> I had the opportunity to establish a flow core last year so here is my
>>> recommendation:
>>> First you need to know what instruments you need and will be needed in
>> the
>>> future.
>>> Establish the floor plan: included future instruments, analysis stations,
>>> office (my office is kind of far of the facility and it's not the most
>>> convenient)
>>> I've included in-house air (it make the room more quiet and instrument
>>> air-compressor will give you back up if in-house air is failing) and fast
>>> internet connection for data transfer to servers.
>>> You need room temperature control, a lab-sink to dump the waste...
>>> I do not have a microscope to check the staining for me.
>>> Do not hesitate to contact me if you have more questions.
>>> Regards,
>>> Nicolas
>>> Nicolas Loof, MS
>>> Manager, CRI Flow Cytometry Shared Facility
>>> Children’s Medical Center Research Institute at UTSW
>>> Biomedical Research Building – NL12.110DB
>>> 6000 Harry Hines Blvd., Dallas, TX 75235
>>> On Apr 10, 2013, at 5:56 PM, Lorelle Parker
>>> <lparker at chori.org<mailto:lparker at chori.org>> wrote:
>>> Hello Fellow Flow-ers,
>>> We are in the process of creating a new flow core from scratch and I was
>>> wondering from the other members of the list:
>>> What changes would you make to your flow core if you could?
>>> What would you have included that you didn't?
>>> Was there anything logistically that you learned afterwards which you
>> wish
>>> you had thought about before?
>>> Do you have a microscope and do users use it to check the staining of
>>> their cells prior to running their samples?
>>> Any additional information would be greatly appreciated.
>>> I thank you all in advance for your time,
>>> Cheers,
>>> Lorelle
>>> Lorelle Parker, M.S., Psy. D.
>>> Flow Cytometry Core
>>> Children's Hospital Oakland Research Institute
>>> 5700 Martin Luther King, Jr. Way
>>> Oakland, California 94609
>>> 510.450.7632
>>> lparker at chori.org<mailto:lparker at chori.org>
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