[Cytometry] Formation of a new core

Nicolas Loof Nicolas.Loof at utsouthwestern.edu
Thu Apr 11 13:56:20 EDT 2013

Hello Lorelle,
I had the opportunity to establish a flow core last year so here is my recommendation:
First you need to know what instruments you need and will be needed in the future.
Establish the floor plan: included future instruments, analysis stations, office (my office is kind of far of the facility and it's not the most convenient)
I've included in-house air (it make the room more quiet and instrument air-compressor will give you back up if in-house air is failing) and fast internet connection for data transfer to servers.
You need room temperature control, a lab-sink to dump the waste...

I do not have a microscope to check the staining for me.

Do not hesitate to contact me if you have more questions.

Nicolas Loof, MS
Manager, CRI Flow Cytometry Shared Facility
Children’s Medical Center Research Institute at UTSW
Biomedical Research Building – NL12.110DB
6000 Harry Hines Blvd., Dallas, TX 75235

On Apr 10, 2013, at 5:56 PM, Lorelle Parker <lparker at chori.org<mailto:lparker at chori.org>> wrote:

Hello Fellow Flow-ers,
We are in the process of creating a new flow core from scratch and I was wondering from the other members of the list:

What changes would you make to your flow core if you could?
What would you have included that you didn't?
Was there anything logistically that you learned afterwards which you wish you had thought about before?
Do you have a microscope and do users use it to check the staining of their cells prior to running their samples?

Any additional information would be greatly appreciated.
I thank you all in advance for your time,



Lorelle Parker, M.S., Psy. D.
Flow Cytometry Core
Children's Hospital Oakland Research Institute
5700 Martin Luther King, Jr. Way
Oakland, California 94609
lparker at chori.org<mailto:lparker at chori.org>

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