[Cytometry] HLA-DR expression on THP-1 monocytes
divekaranagha at yahoo.com
Thu Apr 11 11:39:03 EDT 2013
BioLegend has HLA-DR in several colors. Please check out this link -
Anagha Divekar, PhD
Scientist II, CST and Product Development Team
From: "Sharma, Parul" <Parul_Sharma at dfci.harvard.edu>
To: 'Pam Tallon' <ptallon at nosm.ca>; "cytometry at lists.purdue.edu" <cytometry at lists.purdue.edu>
Sent: Wednesday, April 10, 2013 4:52 PM
Subject: Re: [Cytometry] HLA-DR expression on THP-1 monocytes
Coulter also has an HLA-DR FITC antibody if that helps.
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Pam Tallon
Sent: Tuesday, April 09, 2013 1:35 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] HLA-DR expression on THP-1 monocytes
I was hoping to borrow your expertise on my little problem. I wanted to
measure HLA-DR expression on THP-1 monocytes. I initially used a primary
unconjugated antibody from BD biosciences, with a secondary FITC antibody.
No expression was observed even in a positive control leading us to believe
the issue was with the antibody.
I've been looking to purchase a conjugate HLA-DR Human specific monoclonal
antibody. However the one from BD is quite expensive. I was wondering if
there were any other cheaper companies with high integrity in their
conjugate antibody products, that others have experience with.
I would prefer if the fluorochrome was either PE or FITC .
Research Lab Coordinator - West
Northern Ontario School of Medicine
955 Oliver Road
Thunder Bay, ON
ptallon at nosm.ca
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