[Cytometry] HLA-DR expression on THP-1 monocytes

Anagha Divekar divekaranagha at yahoo.com
Thu Apr 11 11:39:03 EDT 2013

Hi Pam,

BioLegend has HLA-DR in several colors. Please check out this link -

Best luck

Anagha Divekar, PhD
Scientist II, CST and Product Development Team
BioLegend, Inc

 From: "Sharma, Parul" <Parul_Sharma at dfci.harvard.edu>
To: 'Pam Tallon' <ptallon at nosm.ca>; "cytometry at lists.purdue.edu" <cytometry at lists.purdue.edu> 
Sent: Wednesday, April 10, 2013 4:52 PM
Subject: Re: [Cytometry] HLA-DR expression on THP-1 monocytes

Coulter also has an HLA-DR FITC antibody if that helps.

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Pam Tallon
Sent: Tuesday, April 09, 2013 1:35 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] HLA-DR expression on THP-1 monocytes

Good afternoon,

I was hoping to borrow your expertise on my little problem. I wanted to
measure HLA-DR expression on THP-1 monocytes. I initially used a primary
unconjugated antibody from BD biosciences, with a secondary FITC antibody.
No expression was observed even in a positive control leading us to believe
the issue was with the antibody.

I've been looking to purchase a conjugate HLA-DR Human specific monoclonal
antibody. However the one from BD is quite expensive. I was wondering if
there were any other cheaper companies with high integrity in their
conjugate antibody products, that others have experience with.

I would prefer if the fluorochrome was either PE or FITC .

Much thanks,

Pam Tallon

Research Lab Coordinator - West

Northern Ontario School of Medicine

Room MS-3010

955 Oliver Road

Thunder Bay, ON


ptallon at nosm.ca

Office: 807-766-7367

Fax: 807-766-7362

Cell: 807-621-3613

The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

Cytometry mailing list
Cytometry at lists.purdue.edu
Search the list archive at  http://tinyurl.com/cytometry

More information about the Cytometry mailing list