[Cytometry] HLA-DR expression on THP-1 monocytes
Parul_Sharma at dfci.harvard.edu
Wed Apr 10 19:52:30 EDT 2013
Coulter also has an HLA-DR FITC antibody if that helps.
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Pam Tallon
Sent: Tuesday, April 09, 2013 1:35 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] HLA-DR expression on THP-1 monocytes
I was hoping to borrow your expertise on my little problem. I wanted to
measure HLA-DR expression on THP-1 monocytes. I initially used a primary
unconjugated antibody from BD biosciences, with a secondary FITC antibody.
No expression was observed even in a positive control leading us to believe
the issue was with the antibody.
I've been looking to purchase a conjugate HLA-DR Human specific monoclonal
antibody. However the one from BD is quite expensive. I was wondering if
there were any other cheaper companies with high integrity in their
conjugate antibody products, that others have experience with.
I would prefer if the fluorochrome was either PE or FITC .
Research Lab Coordinator - West
Northern Ontario School of Medicine
955 Oliver Road
Thunder Bay, ON
ptallon at nosm.ca
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