[Cytometry] HLA-DR expression on THP-1 monocytes

Sharma, Parul Parul_Sharma at dfci.harvard.edu
Wed Apr 10 19:52:30 EDT 2013


Coulter also has an HLA-DR FITC antibody if that helps.
Parul

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Pam Tallon
Sent: Tuesday, April 09, 2013 1:35 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] HLA-DR expression on THP-1 monocytes

Good afternoon,



I was hoping to borrow your expertise on my little problem. I wanted to
measure HLA-DR expression on THP-1 monocytes. I initially used a primary
unconjugated antibody from BD biosciences, with a secondary FITC antibody.
No expression was observed even in a positive control leading us to believe
the issue was with the antibody.



I've been looking to purchase a conjugate HLA-DR Human specific monoclonal
antibody. However the one from BD is quite expensive. I was wondering if
there were any other cheaper companies with high integrity in their
conjugate antibody products, that others have experience with.



I would prefer if the fluorochrome was either PE or FITC .



Much thanks,



Pam Tallon

Research Lab Coordinator - West



Northern Ontario School of Medicine

Room MS-3010

955 Oliver Road

Thunder Bay, ON

P7B5E1



ptallon at nosm.ca

Office: 807-766-7367

Fax: 807-766-7362

Cell: 807-621-3613


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