[Cytometry] BIM AB for FLow..

Devereaux, Erik Erik.Devereaux at astrazeneca.com
Wed Apr 10 15:06:00 EDT 2013

Hello.. I'm new here... and a Jr. Operator on the AriaII SORP, Canto II.
Does anyone use
A BIM antibody for Flow?

Many Thanks!

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-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Jonathan Cremer
Sent: Wednesday, April 10, 2013 1:56 AM
To: Pam Tallon; cytometry at lists.purdue.edu
Subject: Re: [Cytometry] HLA-DR expression on THP-1 monocytes

Beckman, Miltenyi, eBioscience and BioLegend are a few of the companies
with a large catalog of flow antibodies. Tonbo is a new company which
claims to have cheaper antibodies, but I have no experience with their
products yet.

If you're buying an antibody for the first time and/or you want to test
something, you can easily get big discounts (sometimes 50%) just by
asking. Contact your local sales rep for these companies and see what
he/she can do for you.
Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven

Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811;
3000 Leuven; Belgium

Van: cytometry-bounces at lists.purdue.edu
[cytometry-bounces at lists.purdue.edu] namens Pam Tallon [ptallon at nosm.ca]
Verzonden: dinsdag 9 april 2013 19:34
To: cytometry at lists.purdue.edu
Onderwerp: [Cytometry] HLA-DR expression on THP-1 monocytes

Good afternoon,

I was hoping to borrow your expertise on my little problem. I wanted to
measure HLA-DR expression on THP-1 monocytes. I initially used a primary
unconjugated antibody from BD biosciences, with a secondary FITC
No expression was observed even in a positive control leading us to
the issue was with the antibody.

I've been looking to purchase a conjugate HLA-DR Human specific
antibody. However the one from BD is quite expensive. I was wondering if
there were any other cheaper companies with high integrity in their
conjugate antibody products, that others have experience with.

I would prefer if the fluorochrome was either PE or FITC .

Much thanks,

Pam Tallon

Research Lab Coordinator - West

Northern Ontario School of Medicine

Room MS-3010

955 Oliver Road

Thunder Bay, ON


ptallon at nosm.ca

Office: 807-766-7367

Fax: 807-766-7362

Cell: 807-621-3613

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