[Cytometry] Mice breast tumour sample prep protocol

Meyer, Ernest M. meyere at upmc.edu
Wed Apr 10 11:04:59 EDT 2013

Hi Lola,

The Donnenberg Lab does this all the time: Here are a few references:

Donnenberg VS, E. Michael Meyer and Donnenberg AD.  Multiple Drug Resistance in Cancer Stem Cells. In: Methods in Molecular Biology: Cancer Stem Cells. John Yu, ed. New York: Springer, Humana Press; 2009.

Vera S. Donnenberg, Albert D. Donnenberg, Ludovic Zimmerlin, Rodney J. Landreneau, Rohit Bhargava, Ryan A. Wetzel and Adam M. Brufsky. Localization of CD44 and CD90 positive cells to the invasive front of breast tumors. Clinical Cytometry 2010. 

Ludovic Zimmerlin, Ph.D.., Albert D. Donnenberg, Ph.D., J. Peter Rubin, M.D., Per Basse, M.D., Ph.D., Rodney J. Landreneau, M.D., and Vera S. Donnenberg, Ph.D. Regenerative therapy and cancer: In vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates. Tissue Engineering A, 2010.

Zimmerlin L, Donnenberg VS and Donnenberg AD. Rare Event Detection and Analysis in Flow Cytometry: Bone marrow mesenchymal stem cells, breast cancer stem/progenitor cells in malignant effusions, and pericytes in disaggregated adipose tissue. In: Methods in Molecular Biology: Flow Cytometry Protocols. Howley TS and Howley RG, eds. New York: Springer, Humana Press. 2011.



E. Michael Meyer
Research Scientist
Manager, Flow Cytometry Facility
University of Pittsburgh Cancer Institute
Pittsburgh, PA 15213
(lab) 412.623.3282
meyere at upmc.edu
-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Lola Martinez
Sent: Wednesday, April 10, 2013 10:25 AM
To: purdue Cytometry
Subject: [Cytometry] Mice breast tumour sample prep protocol

Dear all,

     I have a group here that is interested in doing multicolor flow cytometry on cells isolated from mice breast tumor. 

Has anyone out there a protocol to disaggregate those tumours so to get a nice single cell suspension of healthy cells? 

We are planning to try out with collagenase & dispase, but if someone has a protocol that works already we will appreciate it.

Many thanks,


 Biotechnology Program
 Flow Cytometry Unit Head
 Spanish National Cancer Institute (CNIO)
 Melchor Fdez Almagro, 3
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