[Cytometry] HLA-DR expression on THP-1 monocytes

Jonathan Cremer Jonathan.Cremer at med.kuleuven.be
Wed Apr 10 01:56:19 EDT 2013

Beckman, Miltenyi, eBioscience and BioLegend are a few of the companies with a large catalog of flow antibodies. Tonbo is a new company which claims to have cheaper antibodies, but I have no experience with their products yet.

If you're buying an antibody for the first time and/or you want to test something, you can easily get big discounts (sometimes 50%) just by asking. Contact your local sales rep for these companies and see what he/she can do for you.
Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven

Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium

Van: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] namens Pam Tallon [ptallon at nosm.ca]
Verzonden: dinsdag 9 april 2013 19:34
To: cytometry at lists.purdue.edu
Onderwerp: [Cytometry] HLA-DR expression on THP-1 monocytes

Good afternoon,

I was hoping to borrow your expertise on my little problem. I wanted to
measure HLA-DR expression on THP-1 monocytes. I initially used a primary
unconjugated antibody from BD biosciences, with a secondary FITC antibody.
No expression was observed even in a positive control leading us to believe
the issue was with the antibody.

I've been looking to purchase a conjugate HLA-DR Human specific monoclonal
antibody. However the one from BD is quite expensive. I was wondering if
there were any other cheaper companies with high integrity in their
conjugate antibody products, that others have experience with.

I would prefer if the fluorochrome was either PE or FITC .

Much thanks,

Pam Tallon

Research Lab Coordinator – West

Northern Ontario School of Medicine

Room MS-3010

955 Oliver Road

Thunder Bay, ON


ptallon at nosm.ca

Office: 807-766-7367

Fax: 807-766-7362

Cell: 807-621-3613

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