[Cytometry] bunny WBCs
Rachel.Gerstein at umassmed.edu
Tue Apr 9 13:05:50 EDT 2013
I am sure that Katherine Knight's lab could help you:
Rachel M. Gerstein, Ph.D.
Department of Microbiology and Physiological Systems
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-5920 (FAX)
From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] on behalf of Mara Rocchi [Mara.Rocchi at moredun.ac.uk]
Sent: Tuesday, April 09, 2013 9:55 AM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] bunny WBCs
A scientist here would like to use the flow cytometer to do a quick WBC differential on rabbit blood.
I thought about a NH4Cl lysis, maybe one wash and then FSC/SSC, however I am unfamiliar with rabbit flow and don’t seem able to find many publications where I can see a FSC/SSC plot for reference.
Could you please send me some information if you normally flow bunnies? I have only found a reference so far (Davis and Hamilton, J. Vet. Sci (2008), 9(1), 51-66).
P.S. we will also do regular blood smears-just in case-but the number of rabbit on trial is high so something quicker would be better….
Mara Rocchi BVM&S PhD MRCVS
Flow Cytometry Manager
Moredun Research Institute
Pentlands Science Park
Bush Loan, Penicuik
mara.rocchi at moredun.ac.uk
The Moredun Research Institute is a limited company registered in Scotland. Registered number: SC149440
The Moredun Research Institute is a charity registered in Scotland. Registered number: SC022353
Registered office: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, UK
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