[Cytometry] Your flow experiences with Cy3
CLKING at gru.edu
Mon Apr 8 17:18:19 EDT 2013
Cy3 is not so bad in our hands. It's certainly not the brightest fluorophore but as long you are able to resolve it, you're okay. According to the BioLegend spectra analyzer, there's ~15% efficiency of excitation using 488nm laser line and it shows about 80% using 561nm (excitation peak at ~555nm).
Flow Cytometry Core Facility
Georgia Regents University Cancer Center
From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] on behalf of Marjan Gharagozloo [marjangh at gmail.com]
Sent: Friday, April 05, 2013 12:32 PM
Subject: [Cytometry] Your flow experiences with Cy3
There are many controversies in using Cy3 in flow cytometry. Based on
its excitation/ emission spectra, it wouldn't work very well (It has
<30% excitation at 488nm and
virtually none at 561nm).
I'd like to know how it is working in a real experiment. Please share
with me your experiences with Cy3 in a real flowcytomety world!
Marjan Gharagozloo (PhD)
University of Waterloo
School of Pharmacy, PHR3002
10 Victoria St S, N2G 2B2
Kitchener, ON, Canada
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