[Cytometry] Your flow experiences with Cy3

King, William CLKING at gru.edu
Mon Apr 8 17:18:19 EDT 2013

Hi Marjan,
Cy3 is not so bad in our hands. It's certainly not the brightest fluorophore but as long you are able to resolve it, you're okay. According to the BioLegend spectra analyzer, there's ~15% efficiency of excitation using 488nm laser line and it shows about 80% using 561nm (excitation peak at ~555nm).

William King
Flow Cytometry Core Facility
Georgia Regents University Cancer Center

From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] on behalf of Marjan Gharagozloo [marjangh at gmail.com]
Sent: Friday, April 05, 2013 12:32 PM
To: cytometry
Subject: [Cytometry] Your flow experiences with Cy3

Dear all,

There are many controversies in using Cy3 in flow cytometry. Based on
its excitation/ emission spectra, it wouldn't work very well (It has
<30% excitation at 488nm and
virtually none at 561nm).

I'd like to know how it is working in a real experiment.  Please share
with me your experiences with Cy3 in a real flowcytomety world!

Best regards:

Marjan Gharagozloo (PhD)
Postdoctoral Fellow
University of Waterloo
School of Pharmacy, PHR3002
10 Victoria St S, N2G 2B2
Kitchener, ON, Canada
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Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to CLKING at gru.edu. Please update your address book to reflect this change.

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