[Cytometry] Your flow experiences with Cy3

Marjan Gharagozloo marjangh at gmail.com
Fri Apr 5 13:40:04 EDT 2013


Hi Ian,

Thanks for your reply. Do you think Cy3 may work with Bioanalyzer with
two excitation wavelengths (470 nm/630nm) and two emission wavelengths
(525nm/680nm)?

Thanks
Marjan

On 4/5/13, Ian Dimmick <ian.dimmick at newcastle.ac.uk> wrote:
> I have a 532 laser and we find it absolutely fine on our LSR II , have
> used it routinely for CD19 staining and as a general phenotypic marker. We
> have purchased purified antibody and conjugated Cy3 onto it using the
> "lightning link" kits from Innova , this is also working well for us .
>
> Ian
>
> Ian Dimmick
> Flow  Cytometry Core Facility Manager
> Newcastle Upon Tyne University
> Faculty of Medical Sciences
> Bioscience Centre (West Wing)
> International Centre for life
> Newcastle Upon Tyne
> NE1 3BZ
> UK
> Ian.Dimmick at ncl.ac.uk
> CFL Office                           Tel   0044 191 2418831
> CFL Laboratory                Tel   0044 191 241 8825
> Med. School office           Tel   0044 191 222 7155
> Med School Laboratory Tel   0044 191 222 7079
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>
> http://www.ncl.ac.uk/fccf
> http://flowcytometry.calendarhost.com
> <http://flowcytometry.calendarhost.com/>
>
>
>
>
>
>
>
>
>
> On 05/04/2013 17:32, "Marjan Gharagozloo" <marjangh at gmail.com> wrote:
>
>>Dear all,
>>
>>There are many controversies in using Cy3 in flow cytometry. Based on
>>its excitation/ emission spectra, it wouldn't work very well (It has
>><30% excitation at 488nm and
>>virtually none at 561nm).
>>
>>I'd like to know how it is working in a real experiment.  Please share
>>with me your experiences with Cy3 in a real flowcytomety world!
>>
>>--
>>Best regards:
>>
>>Marjan Gharagozloo (PhD)
>>Postdoctoral Fellow
>>University of Waterloo
>>School of Pharmacy, PHR3002
>>10 Victoria St S, N2G 2B2
>>Kitchener, ON, Canada
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