[Cytometry] Anti-CD3 activation for phosflow

Prussin, Calman (NIH/NIAID) [E] CPRUSSIN at niaid.nih.gov
Fri Apr 5 17:40:24 EDT 2013


Flowees!

Has anyone experience with or know of a reference for using Dynabeads® Human T-Activator CD3/CD28 to activate T cells or PBMC for phospho-flow? The phosphorylation events we are looking at (mTOR downstream effectors) take a few hours to occur.

Other then the beads changing cell scatter is there a problem with using them in an LSRII?

 http://products.invitrogen.com/ivgn/product/11161D

Thanks,

Calman
______________________
Calman Prussin
Laboratory of Allergic Diseases
National Institute of Allergy and Infectious Diseases/ NIH
www.tinyurl.com/prussin1



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