[Cytometry] Lung tissue profiling

Alireza Alavi alireza.s.alavi at gsk.com
Mon Jan 30 10:56:41 EST 2012


I have a mouse monoclonal antibody that binds to an extracellular epitope and works well by FACS in cell lines.  It also detects a band in total human lung lysate Western blots.  Can you recommend a staining strategy that would allow us to determine the identity of cells that are positive in the lung (leukocyte, endothelial, epithelial, etc)?  At the moment we are considering co-staining with CD45, CD31, EpCAM.  We have an LSR II.



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