[Cytometry] what is the cells above granulocyte population on PBC of mice?

Steve Bryce sbryce at litronlabs.com
Mon Nov 21 09:07:35 EST 2011


Hongyou,

>From my understanding (and Im not a clinical flow guy), but this area that
you have highlighted would be mainly Neutrophils.
So your three populations would be Lymphocytes, Monocytes, and Neutrophils.

Steve

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Yu, Hongyou
Sent: Thursday, November 17, 2011 5:41 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] what is the cells above granulocyte population on PBC
of mice?

Hi all,

    I run whole peripheral blood staining and lysis RBC with 0.1%
trixton-x100.  I found a population just above the granulocyte in FSS vs SSC
(the rectangle gated). What's it? It seems that they are part of CD11B and
ly6G positive and absolutely CC chemokine receptor 2 (CCR2) negative. Anyone
has suggestions? I attached the picture and I am sure that the circle gated
are granulocyte population. Thank you.

   Kind regards,
   Hongyou
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