[Cytometry] FSC/SSC changes with Annexin V binding buffer
roberta.rigolio at gmail.com
Fri Nov 11 07:14:17 EST 2011
I have a collegue who is evaluating cell death by AV/IP staining in
immortalized neurons on FACS.
We have read healthy untreated cell resuspended in PBS and the same cells
resuspended in AV binding buffer alone.
We observed a decrease in FSC and slight increase in SSC in the binding
buffer resuspended cells vs PBS resuspended ones.
We use a BD kit that works fine on other seceral cell lines.
We hypothized these changes are due to the low Ca2+ concentration in the
Does anyone have similar experience on other cell line or have any
suggestion to explain this strange situation?
Thanks a lot and have a nice week end
PhD in Neuroscience
Dept. Neuroscience and Biomedical Technologies
Via Cadore 48
MI - Italy
Phone: +39 02 64488114
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