[Cytometry] isolating lymphocytes from ethanol-fixed body fluids

wenhuo hu huwenhuo at gmail.com
Tue May 24 19:10:38 EDT 2011


Hi Stephen,

Have you tried any CD3 antibody? Is there any problem. I tried lineage
cocktail it is not big change after fixation.

Hope you are lucky on this.

Wenhuo Hu
MSKCC

On Tue, May 24, 2011 at 5:30 PM, Fang Yao Stephen Hou
<fyshou at uwalumni.com>wrote:

> I appreciate any information regarding available sources of
> antibodies, binding to ethanol-fixed CD3 T lymphocytes. I am planning
> to isolate them either by sorting or immunomagnetic enrichment. Thank
> you in advance.
>
> Stephen Hou
> Marquette University
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