[Cytometry] Cell cycle of activated human PBMC
g.warnes at qmul.ac.uk
Mon May 23 11:13:40 EDT 2011
In my experience naive human T-cells show little detectable cell division, they must zip through the cell cycle very quickly, in this scenario even at 3 days there is little detectable cell division by this method, so I am not surprised by your result. I guess CFSE proliferation gives you more usable results given that the S phase & G2m percentages are at any one time point very low.
Hope this suggestion helps
Dr Gary Warnes
Flow Cytometry Manager
Flow Cytometry Core Facility
Blizard Institute of Cell & Molecular Science
Barts and The London School of Medicine and Dentistry
4 Newark Street
Fax No. 0207-882-2180
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Marjan Gharagozloo
Sent: 22 May 2011 09:53
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Cell cycle of activated human PBMC
I'm working with human PBMC, activated by 2µg/ml CD3/CD28 mAb. I've
performed cell cycle analysis after 24h activation on these cells and I've
got strange histograms! didn't see S phase! I have no idea about the
populations in FSC/SSC dot plot; I can see 3 populations, where I should
gate cells? What is the problem with this test?
Please see attached file. Any suggestion and comment would be greatly
Looking forward your kind reply
Marjan Gharagozloo (Ph.D)
Department of Immunology
School of Medicine
Isfahan University of Medical Sciences
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