[Cytometry] Cell cycle of activated human PBMC

Michael Ormerod m.g.ormerod at btinternet.com
Mon May 23 10:11:06 EDT 2011


How did you prepare the  cells? This can be important with PBLs, 
particularly if you use an ethanolic fixative as the different subsets will 
then take up different amounts of PI.

Regards,

Michael Ormerod,
1 Furze Place,
Furze Hill,
Redhill,
RH1 1ER

Phone: 01737 767879
Mobile: 07802293242

Web site: www.mgormerod.com/
Book: http://flowbook.denovosoftware.com

----- Original Message ----- 
From: "Marjan Gharagozloo" <gharagozlo at sums.ac.ir>
To: <cytometry at lists.purdue.edu>
Sent: Sunday, May 22, 2011 9:52 AM
Subject: [Cytometry] Cell cycle of activated human PBMC


> Dear All,
> I'm working with human PBMC, activated by 2µg/ml CD3/CD28 mAb. I've
> performed cell cycle analysis after 24h activation on these cells and I've
> got strange histograms! didn't see S phase! I have no idea about the
> populations in FSC/SSC dot plot; I can see 3 populations, where I should
> gate cells? What is the problem with this test?
> Please see attached file. Any suggestion and comment would be greatly
> appreciated.
>
> Looking forward your kind reply
>
> Best regards:
> Marjan Gharagozloo (Ph.D)
> Assistant professor
> Department of Immunology
> School of Medicine
> Isfahan University of Medical Sciences
> Isfahan, Iran
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