[Cytometry] Problem with PE compensation in PerCP

Brink, A. A.Brink at vumc.nl
Mon May 23 06:05:48 EDT 2011


Dear everyone,

 

For recent experiments on our BD Aria we wanted to combine a PE labelled
antibody with the viability dye 7AAD.

However, the PE antibody is giving problems: we need a compensation of
more than 100% in the PerCP channel, where we want to measure the 7AAD.
In the last experiment it was even 134%!

 

Does anybody know what can be the cause of this? 

When I take a look at the BD Spectrum viewer, there is hardly any
overlap from PE in PerCP.

 

Best regards,

 

 

 


Arjen Brink
VU University Medical Center

Amsterdam

the Netherlands

 

 



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