[Cytometry] Cell cycle of activated human PBMC

Apostolos Bossios apostolos.bossios at lungall.gu.se
Sun May 22 05:21:05 EDT 2011


Dear Marjan.
I cannot see the plots. 
Best
Apostolos

************************** 
Apostolos Bossios MD, PhD 

University of Gothenburg 
Krefting Research Centre
Box 424
SE-405 30  Gothenburg 
Sweden 
 
Visiting address: Medicinaregatan 1G 
Tel: +46 (0) 31-786 6719
Fax: +46 (0) 31-786 6730
e-mail: apostolos.bossios at gu.se



-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Marjan Gharagozloo
Sent: den 22 maj 2011 10:53
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Cell cycle of activated human PBMC

Dear All,
I'm working with human PBMC, activated by 2µg/ml CD3/CD28 mAb. I've
performed cell cycle analysis after 24h activation on these cells and I've
got strange histograms! didn't see S phase! I have no idea about the
populations in FSC/SSC dot plot; I can see 3 populations, where I should
gate cells? What is the problem with this test?
Please see attached file. Any suggestion and comment would be greatly
appreciated.

Looking forward your kind reply

Best regards:
Marjan Gharagozloo (Ph.D)
Assistant professor
Department of Immunology
School of Medicine
Isfahan University of Medical Sciences
Isfahan, Iran























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