[Cytometry] Cell cycle of activated human PBMC
apostolos.bossios at lungall.gu.se
Sun May 22 05:21:05 EDT 2011
I cannot see the plots.
Apostolos Bossios MD, PhD
University of Gothenburg
Krefting Research Centre
SE-405 30 Gothenburg
Visiting address: Medicinaregatan 1G
Tel: +46 (0) 31-786 6719
Fax: +46 (0) 31-786 6730
e-mail: apostolos.bossios at gu.se
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Marjan Gharagozloo
Sent: den 22 maj 2011 10:53
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Cell cycle of activated human PBMC
I'm working with human PBMC, activated by 2µg/ml CD3/CD28 mAb. I've
performed cell cycle analysis after 24h activation on these cells and I've
got strange histograms! didn't see S phase! I have no idea about the
populations in FSC/SSC dot plot; I can see 3 populations, where I should
gate cells? What is the problem with this test?
Please see attached file. Any suggestion and comment would be greatly
Looking forward your kind reply
Marjan Gharagozloo (Ph.D)
Department of Immunology
School of Medicine
Isfahan University of Medical Sciences
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