[Cytometry] Sorting proliferating cells

Darzynkiewicz, Zbigniew Z_DARZYNKIEWICZ at NYMC.EDU
Wed May 11 14:29:42 EDT 2011

Hello Cindy and "Friends in Cytometry",
It is indeed a very old PNAS paper accessible through:


Another paper: http://onlinelibrary.wiley.com/doi/10.1002/cyto.990010203/pdf


Zbigniew Darzynkiewicz, MD, PhD.
Professor of Pathology and Medicine
New York Medical College
Valhalla, NY.

Department of Pathology
15 Dana Road
Valhalla, NY. 10595
Tell. 914-594-3794, 3780

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Cindy Zuleger
Sent: Wednesday, May 11, 2011 2:04 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Sorting proliferating cells

Hi All- 

I pulled Dr. Darzynkiewicz's answer (below) to a post regarding sorting live proliferating cells from the archives (July 2007).  Does anyone have a specific reference for this in action??  Looking thru the literature on this is daunting, to say the least. 


"Cells that proliferate, especially in S and G2M, have higher RNA and 
protein content and are distinctly larger than non-proliferating cells 
The difference is particularly striking (~ 10 fold in RNA content) if 
one compares quiescent genuine G0- with G1-S-G2M proliferating cells. 
Cell size, thus (volume, forward light scatter) could provide some 
discrimination between live cycling vs non-cycling cells, without a need 
to for fluorochrome-labeling." 

Zbigniew Darzynkiewicz, M.D., Ph.D. 

Professor of Pathology and Medicine 
Director, Brander Cancer Research Institute 
New York Medical College 
BSB, Room 438 
Valhalla, N.Y. 10595 

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