[Cytometry] Sorting proliferating cells

Cindy Zuleger clz at medicine.wisc.edu
Wed May 11 14:04:06 EDT 2011


Hi All- 

I pulled Dr. Darzynkiewicz's answer (below) to a post regarding sorting live proliferating cells from the archives (July 2007).  Does anyone have a specific reference for this in action??  Looking thru the literature on this is daunting, to say the least. 

Thanks, 
Cindy   


"Cells that proliferate, especially in S and G2M, have higher RNA and 
protein content and are distinctly larger than non-proliferating cells 
The difference is particularly striking (~ 10 fold in RNA content) if 
one compares quiescent genuine G0- with G1-S-G2M proliferating cells. 
Cell size, thus (volume, forward light scatter) could provide some 
discrimination between live cycling vs non-cycling cells, without a need 
to for fluorochrome-labeling." 

Zbigniew Darzynkiewicz, M.D., Ph.D. 

Professor of Pathology and Medicine 
Director, Brander Cancer Research Institute 
New York Medical College 
BSB, Room 438 
Valhalla, N.Y. 10595 



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