[Cytometry] primary mouse hepatocytes cell cycle question

Julie Farnsworth jfarnsworth at vcu.edu
Fri May 6 15:25:40 EDT 2011


Could someone help me analyze data from primary mouse hepatocytes 
stained with PI run on BDFACSCanto
I am not sure where to draw the "singlet gate" for the single cells to 
exclude doublets and
not sure what each peak in the histogram represents.
*What are the two smaller peaks?*

I hope you can see the dot plots and histograms below.
Set one shows a larger P1 gate and
the other set shows a smaller/tighter P1 gate.
Which one is correct?

thanks for you help


> Same data file, 2 different gating P1 regions
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