[Cytometry] PE query

Jasneet Kaur Phd2010 jasneetkaur at nii.ac.in
Fri May 6 08:00:53 EDT 2011


thanks all.. i have Aria1 and BD LSR at my disposal. i think invitrogen's
cell tracking dye would be a good idea. i wanted to check it asap thus was
wondering if i can try using PE.

On Fri, May 6, 2011 at 8:19 AM, Nebe-Von-Caron, G <
g.nebe-von-caron at alere.com> wrote:

> Thanks and that is indeed easier if you have the appropriate source.
> Perhaps Jasneet could tell us what equipment he has at his disposal. This
> would perhaps be a general reminder that people might want to  mention when
> raising a question what equipment or ideally the sources and detectors are
> available to them.
>
>
> Gerhard Nebe-von-Caron
> Research Scientist and Biomedical Engineer
> mailto:g.nebe-von-caron at alere.com
>
> Alere International
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> -----Original Message-----
> From: Langsdorf, Chris [mailto:Chris.Langsdorf at lifetech.com]
> Sent: 05 May 2011 17:25
> To: Nebe-Von-Caron, G; Jasneet Kaur Phd2010; cytometry at lists.purdue.edu
> Subject: RE: [Cytometry] PE query
>
> Hi Gerhard and Jasneet,
>
> You might try CellTrace Violet, which has similar cell tracing properties
> to CFSE but emits in the Pacific Blue channel. I have had good success
> labeling populations of lymphocytes with CFSE and CellTrace Violet prior to
> mixing and stimulation.
>
> Regards,
> Chris
>
> -----Original Message-----
> From: cytometry-bounces at lists.purdue.edu [mailto:
> cytometry-bounces at lists.purdue.edu] On Behalf Of Nebe-Von-Caron, G
> Sent: Thursday, May 05, 2011 7:27 AM
> To: Jasneet Kaur Phd2010; cytometry at lists.purdue.edu
> Subject: Re: [Cytometry] PE query
>
> Having got a bit more detail that Jasneet actually wants to label a
> second cell population already having labelled one with CFSE I wonder if
> anyone has tried Carboxynaphthofluorescein succinimidylesther for that.
> Albeit it will only work on dead cells in elevated pH it might still be
> useful if the endpoint could be measured following terminal fixation.
>
>
>
> Gerhard Nebe-von-Caron
> Research Scientist and Biomedical Engineer
>
>
> -----Original Message-----
> From: cytometry-bounces at lists.purdue.edu
> [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Jasneet Kaur
> Phd2010
> Sent: 02 May 2011 05:48
> To: cytometry at lists.purdue.edu
> Subject: [Cytometry] PE query
>
> hi all,
>
> i have a Phycoerythrin dye in my lab (not conjugated to anything)
> obtained
> from Molecular Probes. i have to use a dye to just stain a cell
> population
> with it. does this dye enter/bind to all cell types without any
> preference?
> also, if possible, can anyone send me the protocol for its use? i am
> completely clueless about the concentration/duration of the treatment.
> thanks in advance.
>
> --
> Jasneet Kaur
> c/o Dr. Satyajit Rath
> Research Scholar
> Immunobiology Lab-2
> National Institute of Immunology,
> New Delhi
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-- 
Jasneet Kaur
c/o Dr. Satyajit Rath
Research Scholar
Immunobiology Lab-2
National Institute of Immunology,
New Delhi


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