[Cytometry] Eosinophils auto-florescence

Barry Moran MORANBA at tcd.ie
Fri May 6 05:17:54 EDT 2011


Hi Robert



They are pretty autofluorescent but we've found that eosinophils are fairly easy to identify by SiglecF+ F4/80int CD11bint SSC-hi. If you plot SSC vs SiglecF (in our case on PE) they're fairly clear. We were looking at them in murine peritoneal lavages.



Good luck.



Barry.



Barry Moran

Research Officer and Manager
Flow Cytometry Facility
School of Biochemistry and Immunology
Lab 1.8, Biochemistry Building
Trinity College Dublin
Ireland

Lab  :+ 353  (0)1 896 2761
Mob:+ 353 (0)87 921 0811

barry.moran at tcd.ie<mailto:barry.moran at tcd.ie>

http://www.tcd.ie/Biochemistry/flow/






-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Tweyongyere Robert
Sent: Thursday, May 05, 2011 8:18
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Eosinophils auto-florescence



While analyzing eosinophils for surface markers I have found these cells are

so auto-florescent

Any one who have worked with eousinophils; what is the experience and how do

I go about this situation



--

Robert Tweyongyere

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