[Cytometry] CD34 HSC in normal peripheral blood

D. Robert Sutherland rob.sutherland at utoronto.ca
Thu Mar 31 08:20:32 EDT 2011


Most 'normals' are in the 1-3 per microlitre range, but you occasionally get an outlier who regularly reads in the 6-7 per range.
check out the CSLI H42-A2 document.
best
rob sutherland
University Health Network 
Toronto

On 2011-03-30, at 6:08 PM, Robert Mandle wrote:

> Does anyone have references for the expected number of CD34+ cells in
> non-mobilized normal human blood?  The only referenced number that I have
> found in 0.06% of WBC (Korbling et. al Blood 1995 86:2842) and that is
> pre-ISHAGE Guidelines.  Using the ISHAGE single platform method with PE-CD34
> (BD 550619 clone 563) and Alexa-647 anti-CD45 (BioLegend Clone HI30) we get
> closer to 0.03% of WBC.  Also the BD CD34 TruCount assay insert gives a mean
> of 0.037 % of WBC.  
> 
> 
> 
> Thanks for the help
> 
> Bob
> 
> 
> 
> Robert Mandle
> 
> BioSciences Research Associates
> 
> mandle at bsrlabs.com
> 
> 
> 
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