[Cytometry] Magnetic Bead Plate Washer
KEITH.BAHJAT at providence.org
Wed Mar 16 19:29:58 EDT 2011
We were able to modify our ELX405 to wash Luminex magnetic beads using a couple of off-the-shelf products.
LifeSep 96F – this is a flat magnet that fits into the plate portion of the ELX405. http://www.dextermag.com/lifesep-96f-2501008-1.html
96-well Flexible PVC microplates – BD catalog #353912 - http://www.bdbiosciences.com/ptProduct.jsp?prodId=363772
You have to use the shorter plates or the height of the plate+magnet won't work with the ELX405.
You'll set up a new program that leaves 60 seconds after wash buffer is dispensed. Decrease the force of aspiration and don't let the probe go all the way to the bottom. This will leave the beads on the bottom while the buffer is aspirated. The force of dispensing is sufficient to dislodge them from the magnet for the next wash cycle.
Once we set everything up, it worked like a charm and cost <$500.
Keith Bahjat, Ph.D.
Earle A. Chiles Research Institute
Providence Cancer Center
4805 NE Glisan St, 2N83
Portland, Oregon 97213
keith.bahjat at providence.org
From: Ryan Duggan <rduggan at bsd.uchicago.edu<mailto:rduggan at bsd.uchicago.edu>>
Date: Mon, 14 Mar 2011 13:23:58 -0700
To: "cytometry at lists.purdue.edu<mailto:cytometry at lists.purdue.edu>" <cytometry at lists.purdue.edu<mailto:cytometry at lists.purdue.edu>>
Subject: [Cytometry] Magnetic Bead Plate Washer
If anyone has experience with automated washers used to wash magnetic beads from Luminex Assays, I'd be interested in hearing from you. The two I'm familiar with are the BioTek ELX405, and the Tecan HydroFlex. If you have one of these or something else, please let me know how you like them.
Ryan Duggan, Technical Director
University of Chicago, Flow Cytometry
910 E. 58th, Chicago, IL 60637
Off Ph: 773.702.5582
Lab Ph: 773.702.9212
Email: rduggan at bsd.uchicago.edu<mailto:rduggan at bsd.uchicago.edu>
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