[Cytometry] multicolor kits

Sadia Salam sadiarana at gmail.com
Sat Mar 5 12:07:08 EST 2011


Hi,
Couldn't you use single isotype controls? I did that for many tri-test kits
and it seemed to work.
Regards,
Sadi.

On 4 March 2011 19:44, Shang Shen <shangs at unlv.nevada.edu> wrote:

> Dear flowers,
>
> I am going to run BD Th17/Treg Kit which is with a 3 color (PerCP-Cy5.5, PE
> and Alexa647) mix in one vial. There are no single color controls for
> compensation in this kit. I just can not image how these kind of multicolor
> kits work with flow without compensation involved. Any input would be
> really
> appreciated!
>
> Thanks,
> --
> Shirley
> Genomics Facility; SEB, Rm 3124,
> University of Nevada Las Vegas
> 4505 Maryland PKWY
> Las Vegas, NV 89154-4022
>
> 702-895-1057
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