[Cytometry] cell viabilities at high sort rates?

Vanda Juranic vanda_juranic at yahoo.com
Fri Jan 29 05:13:31 EST 2010

I have such data from several experiments on mouse splenocytes. One is in the attach, post sorted GFP NK cells dyed with PI. Sorted on high speed high pressure, 70 um nozzle. Cells are generally over 95% viable. PI is shown in PerCP Cy5.5 channel (I know it says CD19/CD3 but its NOT, its PI).

Vanda Juranic Lisnic
Department of Histology and Embryology 
Faculty of Medicine, University of Rijeka 
B. Branchetta 20 
51000 Rijeka 
Phone: +385 51 651 170 
Fax: +385 51 651 176

From: Lei Yu <yulei_gt at hotmail.com>
To: Ray Hester <rhester at jaguar1.usouthal.edu>; Cytometry Mailing List <cytometry at flowcyt.cyto.purdue.edu>
Sent: Thu, January 28, 2010 6:37:36 PM
Subject: Re: [Cytometry] cell viabilities at high sort rates?

We are also interested in the same question. We are trying to use Aria II to 
study brain cell injury.

If someone has the data, would you please also send me a copy?

Thanks ahead,

Lei Yu, MD & PhD
Department of Pediatrics
NorthShore University HealthSystem
Evanston, IL 60201
lyu at northshore.org
From: "Ray Hester" <rhester at jaguar1.usouthal.edu>
Sent: Thursday, January 28, 2010 11:13 AM
To: "Cytometry Mailing List" <cytometry at flowcyt.cyto.purdue.edu>
Subject: [Cytometry] cell viabilities at high sort rates?

> Hi,
> Several manufacturers of flow cytometers advertise sort rates of  70,000
> events/particles per sec with purities of >99%, but they don't show (at
> least I haven't seen) viability data of  the cells sorted at these speeds.
> If some lab, commercial or university, has viability data, would you
> send it to us?  We are especially interested in something other than a
> stated percentage or a bar graph, although I'm not sure what that might
> be - perhaps dot plots of  sorted and subsequently 7 AAD-, PI-, or even
> trypan blue-stained, sorted cells could be e-mailed to us?
> Perhaps the type of particle would influence the results, e.g., cellular
> microparticles, bacteria, small lymphocytes, cells from established
> lines, all might behave differently. Nevertheless, we would be
> interested in your results and any particulars - model of flow
> cytometer, nozzle size, etc.
> Thanks for any help with this.
> Ray Hester
> College of Medicine
> The University of South Alabama
> Mobile, AL
> rhester at jaguar1.usouthal.edu
> (251) 460-6029 (ofc)
> _______________________________________________
> Cytometry mailing list
> Cytometry at lists.purdue.edu
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